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目的 :研究哮喘患者的肺炎衣原体感染状况 ,并探讨肺炎衣原体感染在哮喘发病中的作用。 方法 :对 75例哮喘及 6 3例呼吸道感染者进行研究 ,以 10 0例献血员血标本作为健康对照。应用聚合酶链反应 ( PCR)和直接免疫荧光法检测咽拭子标本中的肺炎衣原体 ,以及微量免疫荧光法 ( MIF)检测血清肺炎衣原体 Ig G、Ig M和 Ig A抗体。 结果 :哮喘组肺炎衣原体 Ig G抗体阳性率 ( 81.3% )显著高于献血员组 ( 6 8.0 % ) ,P<0 .0 5 ,与感染组无统计学差异 ( 6 9.8% ) ,P>0 .0 5 ;发作期哮喘组肺炎衣原体感染率 ( 5 9.4% )显著高于呼吸道感染组 ( 34.9% ) ,P<0 .0 5 ;哮喘组肺炎衣原体 Ig G抗体平均滴度 ( 48.38± 6 .94)显著高于呼吸道感染组 ( 2 4.70± 8.77) ,P<0 .0 5 ;但 Ig A抗体平均滴度差异不显著 ;肺炎衣原体感染的哮喘患者中 5 7.1% ( 12 / 2 1)同时存在其他病原体 ;对证实有肺炎衣原体感染的 7例哮喘患者进行抗肺炎衣原体治疗有效。 结论 :肺炎衣原体感染与哮喘的发病密切相关
Objective: To study the status of Chlamydia pneumoniae infection in asthmatic patients and to explore the role of Chlamydia pneumoniae infection in the pathogenesis of asthma. Methods: 75 cases of asthma and 63 cases of respiratory infections were studied, blood samples of 100 donors as a healthy control. Chlamydia pneumoniae was detected by polymerase chain reaction (PCR) and direct immunofluorescence, and serum anti-Chlamydia pneumoniae Ig G, Ig M and Ig A antibodies were detected by micro-immunofluorescence assay (MIF). Results: The positive rate of Chlamydia pneumoniae IgG antibody in asthma group was significantly higher than that in blood donors (81.0% vs 65.0%, P <0.05), but not significantly different from that in infection group (68.8%), P> 0 . The infection rate of Chlamydia pneumoniae in asthma group was significantly higher than that in respiratory tract infection group (34.9%, P <0.05). The mean titer of IgG antibody in asthmatic group was 48.38 ± 6. 94) was significantly higher than that of respiratory tract infection group (4.70 ± 8.77), P <0.05. However, the mean titer of IgA antibody was not significantly different. Among the patients with Chlamydia pneumoniae infection, 51.18% (12/2) Other pathogens were present; anti-Chlamydia pneumoniae treatment was effective in 7 asthmatic patients confirmed as having Chlamydia pneumoniae infection. Conclusion: Chlamydia pneumoniae infection is closely related to the pathogenesis of asthma