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目的研究正己烷接触工人外周血红细胞血影蛋白(Spectrin,Sp)表达的情况,并探讨其作为反应职业性正己烷中毒患者神经损伤生物标志物的可行性。方法以广东省某市某精密玻璃企业8名工人为研究对象,进行职业健康体检,并检测工作场所空气和工作物料中有毒物质的浓度,采用蛋白质免疫印迹(western blot,WB)检测工人外周血红细胞Sp的表达情况。结果正己烷接触工人所在的工作场所中苯、甲苯、二甲苯、正己烷、1,2-二氯乙烷的时间加权平均浓度(Time-weighted average,TWA)均低于职业接触限值,采集的8个物料中有4个物料正己烷相对百分含量分别在0.3%~13.9%之间。4名正己烷接触工人中,有3名出现明显的周围神经损伤,神经-肌电图检查结果提示神经源性损伤,诊断为重度职业性正己烷中毒性周围神经病;4名办公室工人所在工作场所空气中均未检测出正己烷及其同系物和其他职业病危害因素,职业健康体检均未有异常发现。4名正己烷接触工人的外周血红细胞Sp蛋白含量低于办公室工人(P<0.05),且有2例正己烷中毒工人Sp蛋白含量明显降低,结论正己烷中毒工人外周血红细胞中Sp蛋白表达水平降低,提示Sp蛋白可作为反应正己烷所致神经损伤的生物标志物。
Objective To investigate the expression of peripheral blood erythrocyte spectrin (Sp) in n-hexane exposure workers and to explore its feasibility as a biomarker of neurological injury in patients with n-hexane poisoning. Methods Eight workers in a precision glass factory in a certain city of Guangdong Province were taken as the research objects to carry out occupational health examination and to test the concentrations of toxicants in the air and working materials in the workplace. The peripheral blood of workers was detected by western blot (WB) Erythrocyte Sp expression. Results The time-weighted average (TWA) of benzene, toluene, xylene, n-hexane and 1,2-dichloroethane in the workplace where n-hexane was exposed to the workers was below the occupational exposure limit Of the 8 materials in four of the relative content of hexane were between 0.3% to 13.9%. Of the 4 n-hexane exposed workers, 3 showed significant peripheral nerve injury, neuro-EMG results suggestive of neurogenic injury, and were diagnosed with severe occupational n-hexane toxic peripheral neuropathy; 4 office workers at the workplace No detectable n-hexane and its homologues and other occupational hazards were found in the air, and no abnormalities were found in occupational health examination. The sp protein level of peripheral blood erythrocytes of 4 n-hexane exposed workers was lower than that of office workers (P <0.05), and the Sp protein content of 2 n-hexane poisoned workers was significantly lower than that of normal workers. Conclusion The expression level of Sp protein in peripheral blood red blood cells of n-hexane poisoned workers Reduce, suggesting that Sp protein can be used as a biomarker of n-hexane-induced neurological injury.