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为进一步利用野生大豆资源,挖掘大豆荚粒性状关键基因,以黑龙江省大面积种植推广的绥农14为轮回亲本,野生种ZYD00006为供体亲本进行多代回交和自交,得到157株BC3F3株系,对其单株荚数、单株粒数、分枝数、单株粒重和百粒重进行相关性分析,并利用基于混合线性模型的QTL network 2.0软件和遗传搭车原理的卡方测验两种方法进行QTL定位,同时采用QTL network进行了上位性分析。结果表明:单株荚数和单株粒数都与单株粒重、分枝数极显著正相关,与百粒重极显著负相关。用两种方法共检测到4个单株荚数QTL,分别定位在B2,D1a,G和N连锁群上;5个单株粒数QTL,分别定位在F,J,D2和K连锁群上,其中定位在F连锁群上的Satt425~Satt663用两种方法都可以检测到。单株荚数和单株粒数上位性分析均检测到3对互作位点。
In order to further utilize the wild soybean resources and excavate the key genes of soybean pod grain traits, Suinong14, which was popularized by large-scale planting in Heilongjiang Province, was used as the recurrent parent. Wild type ZYD00006 was used as donor parent for multiple generations backcrossing and selfing, and 157 BC3F3 The correlation analysis was made between the pods per plant, the number of per plant, the number of branches, the weight per plant and the weight of hundred kernels. Using the QTL network 2.0 software based on the mixed linear model and the chi square Two methods were tested for QTL mapping, and epistasis was also analyzed using QTL network. The results showed that the number of pods per plant and the number of single plant per plant were significantly and positively correlated with the grain weight per plant and the number of branches, and significantly negatively correlated with the weight per plant. Four QTLs for pod numbers per plant were detected by two methods, which were located on B2, D1a, G and N linkage groups respectively. Five QTLs for grain per plant were mapped on F, J, D2 and K linkage groups , Of which Satt425 ~ Satt663 located in F linkage group can be detected by two methods. The number of pods per plant and grain number epistasis analysis detected three pairs of interaction sites.