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目的建立颈复康颗粒中Z-藁本内酯和洋川芎内酯A的GC含量测定法。方法采用三氯甲烷-水溶剂萃取法提取川芎内酯类成分,采用气相色谱法,HP-5石英毛细管柱(30 m×0.32 mm×0.25μm),FID检测器,程序升温系统进行检测。结果藁本内酯和洋川芎内酯A的线性范围分别是4.095~65.51μg·mL-1(r=0.999 9),2.249~44.98μg·mL-1(r=0.999 8)。回收率分别为96.7%,97.2%。结论该方法简便快速,样品使用量少,可用于颈复康颗粒中Z-藁本内酯和洋川芎内酯A的含量测定。
Objective To establish a GC method for the determination of Z-ligustilide and yangchongxiong lactone A in Jingfukang granules. Methods The constituents of Ligusticum chuanxiong L were extracted by chloroform - water solvent extraction. The constituents were determined by gas chromatography, HP-5 quartz capillary column (30 m × 0.32 mm × 0.25 μm), FID detector and temperature programmed system. Results The linear ranges of ligustilide and yangchongxiong lactone A were 4.095 ~ 65.51μg · mL-1 (r = 0.999 9) and 2.249 ~ 44.98μg · mL-1 (r = 0.999 8) respectively. The recoveries were 96.7% and 97.2% respectively. Conclusion The method is simple and rapid, with less sample and can be used to determine the contents of Z-ligustilide and yangchongxiong lactone A in Jingfukang granules.