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目的探讨湖北钉螺滇川亚种在垸内水网地区生存繁殖的可能性和期望生存时间。方法采用笼养法,将采自四川省丹棱县的湖北钉螺滇川亚种、湖北省江陵县实验区内的湖北钉螺指名亚种进行雌雄鉴定分离,分别以雌雄比1∶1配对后,放养在江陵县实验区的螺笼内,分别于3、6个月和9个月后,定期观察其生存和繁殖情况。将采集的滇川亚种钉螺采用室内瓷盘饲养法饲养,采用动物生存寿命表法计算滇川亚种钉螺在脱离原孳生环境后的期望生存时间。结果滇川亚种钉螺在同种及混合现场放养状态下,经过3个月和6个月后,存活率>75%,至9个月后仍有20%以上的存活率。两个亚种钉螺3个月的存活率差异有统计学意义(23χ个月=38.641,P<0.05),同一亚种雌螺与不同亚种雄螺放养后,雌螺存活率差异有统计学意义(2aχf=13.255,P<0.05;2cχg=4.882,P<0.05);9个月后各组均可查获数量不等的子代钉螺。室内实验推算滇川亚种钉螺和指名亚种钉螺的期望生存时间分别为35.84 d和41.16 d。结论滇川亚种钉螺离开原孳生环境后有足够的存活时间完成远距离被动迁移;迁入水网地区后能够继续生存繁殖,并能产生子代。
Objective To explore the possibility and survival time of survival and reproduction of Snail Spinachia in Hubei embankment. Methods The captive method was used to identify the male and female Snail snails in Hubei Snail, Yunnan and Sichuan subspecies collected from Danling County in Sichuan Province and Jiangling County in Hubei Province. After mating with male and female ratio of 1: 1, Stocking in Jiangling County experimental area of the cage, respectively, 3,6 months and 9 months after the regular observation of their survival and reproduction. The collected Snakehead molluscum was kept in indoor porcelain plate feeding method, and the life expectancy was calculated using the method of animal life table. Results After surviving for 3 months and 6 months, the survival rate of A. japonicus was higher than 75% at the same species and mixed stocking sites. After 9 months, the survival rate was still above 20%. The survival rates of three subspecies snails at 3 months were statistically significant (23 × months = 38.641, P <0.05). There was a statistically significant difference in the survival rate of female snails between the female and the female subspecies of the same subspecies Significance (2aχf = 13.255, P <0.05; 2cχg = 4.882, P <0.05). After 9 months, snails of different sizes could be detected in all groups. The expected survival time of in vitro snail and the designated subspecies snail in Yunnan were 35.84 days and 41.16 days, respectively. Conclusion The Asian snakes of Mythimna separata have enough survival time to complete long-distance passive migration after they leave the original breeding environment. After moving into the water network, they can continue to survive and multiply, and can produce offspring.