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对1 432株海洋和极地来源的细菌进行抗南方根结线虫的离体筛选,并对其中活性较好的一株菌进行菌种鉴定并通过盆栽实验验证其在实际生防中的效果.设计诱导系统抗性实验,测定植物防御酶活的变化,初步研究细菌的抗虫机制.结果显示,从1 432株细菌离体筛选得到了9株具有抗南方根结线虫的细菌,其中南极土壤来源的菌株1A00316在离体和活体中均表现出较好的抗性,其生防效果达到71.67%,经鉴定该菌株为恶臭假单胞菌(Pseudomonas putida).诱导番茄系统抗性实验结果显示,经菌株1A00316发酵液处理后的番茄3种防御酶苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)以及过氧化物酶(POD)的酶活均显著提高,分析数据认为该菌株的菌体可诱导番茄系统抗性.本研究表明恶臭假单胞菌1A00316抗南方根结线虫效果较好,可作为微生物制剂应用于实际生防中;该菌不仅有直接抗虫作用,还存在间接抗虫机制,其多重抗虫机制也具有进一步的研究价值.
A total of 1 432 strains of marine and polar origin bacteria were screened against root-knot nematode in vitro, and one strain with better activity was identified and tested in pot experiment to verify its effectiveness in actual biocontrol. Inducing system resistance test and determining the change of plant defense enzyme activity, and preliminary study on the mechanism of bacterial resistance.The results showed that 9 strains of bacteria with resistance to Meloidogyne incognita were screened from 1 432 strains of bacteria, of which Antarctic soil source Of strain 1A00316 showed good resistance both in vitro and in vivo, and the biocontrol effect was 71.67%, which was identified as Pseudomonas putida.The results of inducible tomato system resistance showed that, The activity of three defense enzymes, PAL, PPO and POD in the tomato broth treated with strain 1A00316 was significantly increased. According to the analysis data, The strain of bacteria can induce tomato system resistance.The present study shows that Pseudomonas putida 1A00316 against root-knot nematode better effect, can be used as a microbial agent in the actual biological control; the bacteria not only has a direct insect-resistant effect, but also Indirect Worm mechanism, its multiple mechanisms of insect has also further research value.