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目的:探讨人参皂苷Rg1、Re、Rb1在人参药材中主根、芦头、参须的富集情况,以确保人参中以上3种人参皂苷资源被充分利用,为开发人参各部位的临床应用提供科学依据。方法:采用高效液相色谱法测定产自于吉林通化的生晒参各部位中人参皂苷Rg1、Re和Rb1的含量。色谱条件为:安捷伦1200系列高效液相色谱仪;安捷伦ZORBAX Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm);柱温25℃;检测波长为203nm;流速为0.7mL·min-1,乙腈-水梯度洗脱。结果:人参参须含有较高的人参皂苷Re(0.745 1%)、Rb1(0.859 6%);而人参芦头含有较高的人参皂苷Rg1(0.415 8%)。结论:针对性地提取人参参须、芦头中的人参皂苷Rg1、Re和Rb1单体,应能提高单体化合物的提取效率,更好地服务于创新药物的开发。
OBJECTIVE: To investigate the enrichment of ginsenosides Rg1, Re, Rb1 in Radix Ginseng, and to make sure that the above three kinds of ginsenosides in Ginseng are fully utilized and provide scientific basis for the clinical application of ginseng in accordance with. Methods: The content of ginsenoside Rg1, Re and Rb1 in raw sun-dried ginseng from Jilin Tonghua was determined by high-performance liquid chromatography. The chromatographic conditions were: Agilent 1200 Series HPLC; Agilent ZORBAX Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm); column temperature 25 ° C .; detection wavelength 203 nm; flow rate 0.7 mL · min -1, Water gradient elution. Results: Ginsenosides should contain high concentrations of ginsenoside Re (0.745 1%) and Rb1 (0.859 6%), while ginseng root contains higher ginsenoside Rg1 (0.415 8%). Conclusion: The ginsenoside Rg1, Re and Rb1 in Radix Ginseng should be extracted from ginseng, and the extraction efficiency of monomeric compounds should be improved to better serve the development of innovative drugs.