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目的:探讨并研究磁性纳米微粒对不同种生物样品中蛋白质的分离方法及效果。方法:磁性纳米微粒与AEAPS反应后,表面修饰并连接-NH2制成蛋白质分离器,通过氨基与羧基的结合及外磁力作用分离蛋白质。结果:MNP-NH2对BSA的结合呈现直线相关(r=0.9942,p<0.001)。对几种来源不同的生物样品中可溶性蛋白的分离结果显示,各实验组数值与对照组比较,均具有显著性差异(P<0.05～0.001)。结论:MNP-NH2对于分离生物样品中蛋白质具有较好的分离效果对蛋白质活性影响较小。
Objective: To explore and study the method and effect of magnetic nanoparticles on the separation of proteins in different biological samples. Methods: The magnetic nanoparticles were reacted with AEAPS, surface modified and linked with -NH2 to form a protein separator, and the proteins were separated by the binding of amino group and carboxyl group and external magnetic force. RESULTS: The binding of MNP-NH2 to BSA showed a linear correlation (r=0.9942, p<0.001). The results of the separation of soluble proteins from different sources of biological samples showed that there was a significant difference between the values of the experimental groups and the control group (P<0.05-0.001). Conclusion: MNP-NH2 has good separation effect on the separation of proteins in biological samples and has little effect on protein activity.