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测定生物尿中氮量是评估生物代谢功能、研究生物能量转化的一个重要指标.目前尿氮的测定主要是微量克氏定过法,但这种方法操作繁琐、费时.我们在进行棘腹蛙生态能流模型的研究中,采用脲酶先将尿液中的尿素水解成氨,然后利用氨与奈氏试剂作用显色后,在420nm波长处测量吸光度.其摩尔吸光系数为1.2×10~4,氮含量在10～15μg/50ml范围内符合比耳定律.本法具有简便、快速、准确等特点.可直接用于生物尿样中氮含量的测定.1 试验部分1.1 仪器与试剂721型分光光度计(上海第三分析仪器厂)奈氏试剂:取KI80g.HgI_2115g和NaOH1g分别溶于水后混合在一起,用水定容至500ml,贮于棕色瓶中.
Determination of nitrogen in biological urine is to assess the biological function of metabolism, bioenergy conversion study of an important indicator of the current determination of urine nitrogen is mainly micro Kjeld set method, but this method is cumbersome and time-consuming operation of our In the study of eco-energy model, urease was used to hydrolyze urea in urine to ammonia first, then absorb the light at the wavelength of 420 nm by using ammonia and Nessler’s reagent, and the molar absorptivity was 1.2 × 10 -4 , Nitrogen content in the range of 10 ~ 15μg / 50ml Beer’s law in line with this method is simple, fast and accurate and can be directly used for the determination of nitrogen content in biological urine samples.1 Experimental 1.1 Instruments and reagents 721-type spectroscopy Photometer (Shanghai Third Analytical Instrument Factory) Nessler’s reagent: take KI80g.HgI_2115g and NaOH1g were dissolved in water, respectively, mixed together, the volume of water to 500ml, stored in a brown bottle.