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目的探讨着色性干皮病基因D(XPD)单核苷酸多态性与铂类药物化疗后肾功能变化的关系。方法化疗前采集80例恶性肿瘤患者外周静脉血,采用多聚酶链反应-限制性长度多态性分析进行XPD Lys751Gln基因多态性分型,观察不同分型中铂类药物化疗前后肾功能变化情况。结果 80例患者中,2例(2.5%)携带Gln/Gln纯合基因,50例(62.5%)携带Lys/Lys纯合基因,28例(35.0%)携带Lys/Gln杂合基因。各种XPD基因型化疗后肌酐水平、化疗后肌酐水平增加绝对值、化疗后肌酐水平增加相对值比较差异均无统计学意义(P>0.05)。结论 XPD Lys751Gln基因分型与铂类药物化疗后肾功能下降无明显相关性,不能预测铂类药物化疗后肾功能下降程度。
Objective To investigate the relationship between single nucleotide polymorphism of chromosomal dry skin disease gene D (XPD) and renal function after platinum-based chemotherapy. Methods Peripheral venous blood was collected from 80 patients with malignant tumor prior to chemotherapy. The polymorphism of XPD Lys751Gln was genotyped by polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP). The changes of renal function before and after platinum - based chemotherapy were observed. Results Of the 80 patients, 2 (2.5%) carried Gln / Gln homozygous genes, 50 (62.5%) carried Lys / Lys homozygous genes and 28 (35.0%) carried Lys / Gln heterozygous genes. Creatinine levels after chemotherapy for various XPD genotypes, creatinine levels after chemotherapy were increased absolute values, and there was no significant difference in relative creatinine levels after chemotherapy (P> 0.05). Conclusions There is no significant correlation between XPD Lys751Gln genotyping and renal function decline after platinum drug chemotherapy, and it can not predict the degree of renal function decline after platinum drug chemotherapy.