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目的:阐明γ干扰素抑制血管内膜增生和防治再狭窄的体内机制。 方法:建立兔再狭窄血管模型。将60只新西兰纯种兔分为实验组(n= 30)术前1 天给予重组γ干扰素和对照组(n=30)术前不给干扰素。每组分别于术后1周、2 周和4周处死动物,每次10只。动态观察给予重组γ干扰素对损伤后不同时期内膜血管平滑肌细胞(VSMC)原位表达c-m yc信使核糖核酸(mRNA)和增殖细胞核抗原的影响。 结果:γ干扰素显著抑制1 周和4周时内膜VSMC增殖细胞核抗原的表达,抑制率分别为88.50% 和58.89% ;显著抑制新生内膜VSMCc-m ycm RNA的表达,抑制率分别为1周91.45% 、2 周93.66% 和4 周52.92% 。 结论:γ干扰素抑制内膜VSMC增殖和再狭窄伴c-m yc癌基因表达的下调,为防治再狭窄提供了一条途径
OBJECTIVE: To elucidate the mechanism of interferon gamma in inhibiting intimal hyperplasia and preventing restenosis. Methods: Rabbit restenosis vascular model was established. Sixty New Zealand purebred rabbits were divided into experimental group (n = 30), given recombinant interferon gamma and control group (n = 30) one day before surgery, and no interferon was given before operation. Animals in each group were sacrificed at 1 week, 2 weeks and 4 weeks after operation, 10 animals each. The effect of recombinant interferon gamma on in situ expression of c-m yc messenger RNA (mRNA) and proliferating cell nuclear antigen (PCNA) in VSMCs was observed dynamically. RESULTS: Interferon gamma significantly inhibited the expression of proliferating cell nuclear antigen (PCNA) in VSMCs at 1 week and 4 weeks, with inhibitory rates of 88.50% and 58.89%, respectively. It significantly inhibited the expression of neointimal VSMCc-m ycm RNA and inhibited Rates were 91.45% for 1 week, 93.66% for 2 weeks and 52.92% for 4 weeks, respectively. CONCLUSION: IFN-γ inhibits the down-regulation of c-Myc oncogene expression in VSMCs with proliferation and restenosis in intima, which provides a way to prevent restenosis