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目的:探讨ERβ和ERK1/2在卵巢上皮性癌组织中的表达及其相关性。方法:采用RT-PCR检测70例卵巢上皮性癌、24例卵巢良性肿瘤、24例正常卵巢组织中ERβ和ERKl/2 mRNA的表达,并分析其与临床病理特征之间的关系及两者的相关性。结果:卵巢上皮性癌组织中ERβ阳性表达率为55.7%,显著低于卵巢良性肿瘤(91.7%)及正常卵巢组织(100.0%),P<0.05;ERK1与ERK2的阳性表达率分别为85.7%和85.7%,显著高于卵巢良性肿瘤(50.0%和50.0%)及正常卵巢组织(33.3%和33.3%),P<0.05;卵巢良性肿瘤与正常卵巢组织相比,ERβ和ERK1/2表达差异无统计学意义,P>0.05。ERβ阳性表达率与组织学类型、组织分化程度及淋巴转移相关,P<0.05;与患者年龄、FIGO分期及腹水无关,P>0.05。ERK1/2阳性表达率与组织分化程度、FIGO分期和腹水相关,P<0.05;与患者年龄、组织学类型及淋巴转移无关,P>0.05。在卵巢上皮性癌组织中,ERβ和ERK1/2表达呈负相关,r值分别为-0.743和-0.832,P值分别为0.009和0.001。结论:卵巢上皮性癌组织中ERβ表达的降低和ERK1/2的过高表达可能在卵巢癌发生、发展和侵袭中有协同作用,这可能为卵巢癌的预后和内分泌治疗提供新的靶点和方向。
Objective: To investigate the expression of ERβ and ERK1 / 2 in ovarian epithelial carcinoma and their correlation. Methods: The expressions of ERβ and ERK1 / 2 mRNA in 70 cases of ovarian epithelial carcinoma, 24 cases of benign ovarian tumor and 24 cases of normal ovarian tissue were detected by RT-PCR. The relationship between ERβ and ERK1 / 2 mRNA and clinicopathological characteristics Correlation. Results: The positive rate of ERβ expression in ovarian epithelial carcinoma was 55.7%, which was significantly lower than that in benign ovarian tumor (91.7%) and normal ovarian tissue (100.0%), P <0.05. The positive rates of ERK1 and ERK2 were 85.7% And 85.7%, respectively, which were significantly higher than those in benign ovarian tumors (50.0% and 50.0%) and normal ovarian tissues (33.3% and 33.3%, P <0.05). The expression of ERβ and ERK1 / 2 in benign ovarian tumors was significantly lower than that in normal ovarian tissues No statistical significance, P> 0.05. The positive expression rate of ERβ was correlated with histological type, histological differentiation and lymphatic metastasis (P <0.05), but not with age, FIGO stage and ascites (P> 0.05). The positive expression rate of ERK1 / 2 was correlated with the degree of differentiation, FIGO stage and ascites, P <0.05, but not with age, histological type and lymphatic metastasis (P> 0.05). In epithelial ovarian cancer, ERβ and ERK1 / 2 expression was negatively correlated, r values were -0.743 and -0.832, P values were 0.009 and 0.001. CONCLUSION: The decrease of ERβ expression and overexpression of ERK1 / 2 in epithelial ovarian cancer may play a synergistic role in the occurrence, development and invasion of ovarian cancer, which may provide a new target for the prognosis and endocrine therapy of ovarian cancer. direction.