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Objective: To investigate the clinical efficacy of transcutaneous electrical acupoint stimulation combined with propofol infusion during the treatment of patients under colonoscopy. Methods: A total of 90 patients received painless colonoscopy were randomly divided into three groups: transcutaneous electrical acupoint stimulation combined with propofol group (group T), sufentanil combined with propofol group (group S) and propofol group (group P), with 30 cases in each group. The patients in group T were anesthetized with propofol after transcutaneous electrical acupoint stimulation of 30min at Hegu (LI4), Neiguan (PC6), Zusanli (ST36), Shangjuxu (ST37) and Sanyinjiao (SP6) on both sides, and the electrical stimulation lasted until the end of the operation. Group S and group P were pasted electrode slices at the same acupoint with group T, but no electrical stimulation was given. The other treatments were the same as those in group T. Group S was given sufentanil 0.1ug/kg at the beginning of anesthesia. The levels of blood pressure (MAP), heart rate (HR) and pulse oxygen saturation (SpO2) were continuously monitored at time points of entering operating room (T0), disappearance of eyelash reflex (T1), during operation (through the liver region T2) and immediately after operation (T3), and the total dosage of propofol, awakening time and incidence of adverse reactions in each group were recorded. Results: Compared with group P, the blood pressure and heart rate of the patients at T1 and T2 in group T were more stable (P<0.05), the awakening time after operation was significantly shorter (P<0.05), and the dosage of propofol was significantly lower than that in group P (P<0.05). The incidence of bradycardia, hypotension and respiratory depression decreased significantly (P<0.05). Compared with group S, the postoperative awakening time of group T was significantly shorter (P<0.05), and there was no significant difference in other indexes. Conclusion: Transcutaneous electrical acupoint stimulation is effective during the treatment of analgesia under colonoscopy. It can reduce the dosage of anesthetics, reduce the incidence of postoperative adverse reactions and shorten the awakening time of patients.