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目的:研究黄芪丹参不同配伍对气虚血瘀证大鼠血液流变学和血管内皮因子的影响。方法:将SD大鼠随机分为对照组、模型组、丹参组、黄芪组、黄芪丹参1:1组、2:1组、4:1组,采用限食、游泳、皮下注射肾上腺素方法建立气虚血瘀证大鼠模型,对照组与模型组给予蒸馏水,用药组分别给予不同剂量的丹参、黄芪及不同比例黄芪丹参配伍灌胃给药治疗,连续27日。测定血液流变学指标,内皮素-1(ET-1)、一氧化氮(NO)、血栓素B2(TXB2)和6-酮-前列腺素-F1α(6-keto-PGF1α)含量。结果:模型组大鼠血液流变学参数和血管内皮因子与对照组比较,差异具有统计学意义(P<0.01)。与模型组比较,黄芪丹参1:1组、2:1组、4:1组血液流变学参数显著降低(P<0.05或P<0.01),NO、6-keto-PGF1α、6-keto-PGF1α/TXB2明显升高(P<0.01),ET-1、TXB2明显降低(P<0.01)。与1:1组比较,2:1组血液流变学参数显著下降(P<0.05或P<0.01),NO、6-keto-PGF1α、6-keto-PGF1α/TXB2显著升高(P<0.05或P<0.01),TXB2明显降低(P<0.05)。2:1组大鼠血液流变学参数和血管内皮因子与4:1组比较,差异具有统计学意义(P<0.05或P<0.01)。结论:黄芪丹参配伍对气虚血瘀证大鼠模型具有改善血液流变及保护血管内皮作用,其中以黄芪丹参2:1组最佳。
Objective: To study the effect of different compatibility of Radix Astragali and Radix Salviae Miltiorrhizae on hemorheology and vascular endothelial factor in rats with Qi-deficiency and Blood-stasis Syndrome. Methods: SD rats were randomly divided into control group, model group, Salvia miltiorrhiza group, Astragalus group, Astragalus root group 1: 1 group, 2: 1 group and 4: 1 group. Qi deficiency blood stasis syndrome rat model, the control group and model group were given distilled water, the treatment group were given different doses of Salvia, Astragalus and different proportions of Radix Salviae Gynostemma medication administration, for 27 consecutive days. The indexes of hemorheology, ET-1, NO, TXB2 and 6-keto-PGF1α were determined. Results: Compared with the control group, the hemorrheological parameters and vascular endothelial factor in the model group were significantly different (P <0.01). Compared with the model group, the parameters of hemorheology of 1: 1, 2: 1, 4: 1 group were significantly lower (P <0.05 or P <0.01), NO, 6-keto-PGF1α, PGF1α / TXB2 was significantly increased (P <0.01), ET-1, TXB2 was significantly decreased (P <0.01). Compared with the 1: 1 group, the parameters of hemorheology in 2: 1 group were significantly decreased (P <0.05 or P <0.01), NO, 6-keto-PGF1α and 6-keto-PGF1α / TXB2 were significantly increased Or P <0.01), TXB2 was significantly lower (P <0.05). Compared with the 4: 1 group, the difference of hemorrheological parameters and vascular endothelial factor in 2: 1 group was statistically significant (P <0.05 or P <0.01). Conclusion: Radix Astragali and Radix Salviae Miltiorrhizae compatibility can improve blood rheology and protect vascular endothelium in rats with Qi-deficiency and Blood-stasis syndrome model, among which Radix Salviae Miltiorrhizae 2: 1 group is the best.