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探讨促性腺激素释放激素（GnRH）及其受体表达与肝癌病理学分级和肝癌细胞增殖的关系．方法：用常规组织学HE染色确定肝癌的病理学分级；用免疫组织化学SABC技术检测49例肝细胞性肝癌（HCC）中增殖细胞核抗原（PCNA），GnRH及其受体的表达．结果：GnRH及其受体均定位于肝癌细胞的胞膜和胞浆．Ⅰ级～Ⅱ级肝癌组织GnRH的阳性率为84．6％（20／23），显著高于Ⅲ级肝癌47．1％（8／17）的阳性率（P＜0．05）和Ⅳ级肝癌11．1％（1／9）的阳性率（P＜0．01）．Ⅰ级～Ⅱ级肝癌组织GnRH受体的阳性率为91．1％（21／23），明显高于Ⅲ级肝癌41．2％（7／17）的阳性率（P＜0．01）和Ⅳ级肝癌11．1％（1／9）的阳性率（P＜0．01）．GnRH阳性肝癌的PCNA标记指数（20．4±12．1）％低于GnRH阴性肝癌（39．1±25．3）％（P＜0．05）；GnRH受体阳性肝癌的PCNA标记指数（19．2±13．5）％亦明显低于GnRH受体阴性肝癌（40．5±29．1）％（P＜0．01）．结论：GnRH可能参与肝癌细胞的增殖和分化的调节．
To investigate the relationship between the expression of gonadotropin-releasing hormone (GnRH) and its receptor and the pathological grade of hepatocellular carcinoma and the proliferation of hepatoma cells. METHODS: The pathological grade of liver cancer was determined by HE histological staining. The expression of proliferating cell nuclear antigen (PCNA), GnRH and its receptor were detected in 49 cases of hepatocellular carcinoma (HCC) by immunohistochemical SABC technique. RESULTS: GnRH and its receptors were localized in the membrane and cytoplasm of hepatoma cells. The positive rate of GnRH in grade I-II liver cancer tissue was 84.6% (20/23), which was significantly higher than that of grade III liver cancer 47.1% (8/17) (P<0.05) and grade IV. The positive rate of liver cancer was 11.1% (1/9) (P<0.01). The positive rate of GnRH receptors in grade I-II liver cancer tissues was 91.1% (21/23), which was significantly higher than that of grade 4 liver cancer (41.2%, 7/17) (P<0.01). The positive rate of 11.1% (1/9) of grade IV liver cancer (P<0.01). The PCNA labeling index (20.4±12.1)% in GnRH-positive liver cancer was lower than that in GnRH-negative liver cancer (39.1±25.3)% (P<0.05); the PCNA labeling index of GnRH receptor-positive liver cancer ( 19.2±13.5)% was also significantly lower than GnRH receptor-negative HCC (40.5±29.1)% (P<0.01). Conclusion: GnRH may be involved in the regulation of proliferation and differentiation of hepatoma cells.