根据文献报道及作者实验中的体会 ,对人肿瘤细胞悬液裸鼠皮下接种方法及影响移植成功的因素从瘤细胞、裸鼠和操作者等三方面进行综述。较好地认知人肿瘤细胞悬液裸鼠皮下接种的具体操作程序和影响移植是否成功的多方面因素 ,为人肿瘤裸鼠皮下移植模型成功建立提供参考 According to the reports in the literature and the experience of the authors in the experiment, the methods of subcutaneous inoculation of human tumor cell suspension in nude mice and the factors affecting the success of transplantation are reviewed from three aspects: tumor cells, nude mice and operators. A better understanding of the specific procedure of subcutaneous inoculation of human tumor cell suspension in nude mice and the multifactorial factors that influence the success of transplantation are the reference for the successful establishment of subcutaneous transplantation model of human tumor nude mice