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目的：探讨重组人内皮抑制素对 ＴＮＦα和ＩＬ－８介导的人血管内皮细胞株（ｅｎｄｏｔｈｅｌｉａｌ ｃｅｌｌ ｏｆ ｖｅｓｓｅｌｓ，ＥＣＶ）细胞增殖的抑制作用，并观察内皮抑制素对ＥＣＶ表面粘附分子表达的影响。方法：用ＭＴＴ法测定ＥＣＶ的增殖；用间接免疫荧光法，在流式细胞仪上测定 ＥＣＶ表面粘附分子的表达。结果：内皮抑制素浓度在 １００～１００００ ｎｇ／ｍｌ时，能显著抑制ＥＣＶ的增殖（Ｐ＜０．０１），并抑制ＩＬ－８和ＴＮＦα介导的ＥＣＶ增殖，且呈剂量依赖性关系。内皮抑制素在 １００～１０００ ｎｇ／ｍｌ时可抑制内皮细胞表面ＣＤ６２Ｅ、ＣＤ４０等粘附分子的表达。结论：内皮抑制素不但可抑制血管内皮细胞的增殖，控制肿瘤新生血管的形成，而且可抑制血管内皮细胞表面粘附分子的表达，对肿瘤的转移亦可能起抑制作用。
Objective: To investigate the inhibitory effect of recombinant human endostatin on the proliferation of human vascular endothelial cells (ECV) induced by TNFα and IL-8, and to observe the effect of endostatin on the expression of adhesion molecules on ECV surface. . Methods: The proliferation of ECV was measured by MTT assay; the expression of ECV surface adhesion molecules was measured by flow cytometry using indirect immunofluorescence. Results: When the endostatin concentration was 100 to 10000 ng/ml, ECV proliferation was significantly inhibited (P<0.01), and IL-8 and TNFα-mediated ECV proliferation were inhibited in a dose-dependent manner. Endostatin inhibited the expression of adhesion molecules such as CD62E and CD40 on the surface of endothelial cells at 100-1000 ng/ml. Conclusion: Endostatin can not only inhibit the proliferation of vascular endothelial cells and control the formation of tumor neovascularization, but also inhibit the expression of adhesion molecules on the surface of vascular endothelial cells, and may also inhibit the metastasis of tumors.