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通过组培方法从374粒椪柑种子培养中获得449株小苗。利用流式细胞仪进行染色体倍性分析,发现其中7株小苗为四倍体植株。采用13对SSR引物分析表明这7株四倍体植株均来自珠心细胞,为同源四倍体。这些四倍体的获得为下一步培育三倍体无核品种奠定了基础。
A total of 449 seedlings were obtained from 374 seedlings of Cynanchum Komarovii through tissue culture. Chromosome ploidy analysis using flow cytometry and found that seven of the seedlings tetraploid plants. Thirteen pairs of SSR primer analysis showed that the seven tetraploid plants were from the heart of the cells, the autotetraploid. The availability of these tetraploids laid the foundation for the further cultivation of triploid seedless varieties.