通常测定白细胞移动抑制因子(LIF)有两种方法,即一步法和两步法(或称直接法和间接法)。人们认为一步法省时,但由于抗原或有丝分裂原的存在能影响移动结果,因而敏感性差.二步法虽比一步法敏感,但费时而且操作繁琐。本文介绍一种间接测定 LIF 的一步法。该法操作简便,可能在24小时内获得结果。敏感性好,用血量较少,有丝分裂原 There are usually two ways to measure leukocyte migration inhibitory factor (LIF), one-step and two-step (also known as direct and indirect). One-step method is thought to save time, but the sensitivity is poor because of the presence of antigen or mitogen can affect the moving result, although the two-step method is more sensitive than one-step method, it is time-consuming and cumbersome to operate. This article describes a one-step method for indirect determination of LIF. The method is easy to operate and may yield results within 24 hours. Sensitive, with less blood, mitogen