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光子计数成像系统可以探测生物超微弱发光 ,但是只能探测生物的宏观图像 ,若要深入到细胞、分子水平 ,必须有显微光子计数成像系统。二者的区别在于显微光子计数成像系统是噪声受限系统。本文报道的显微光子计数成像系统 ,采用14 C同位素光源来监测系统的状态 ,保证实现极限探测。该系统可以用来研究痕量生物分子的分布和功能 ,显示钙离子在细胞内外的分布 ,活性氧、基因表达的监测等。由单光子到单分子、组织学图像到功能图像的转变 ,将是重要的发展 ,为生物学医学的应用提供了光明的前景。
Photon counting imaging system can detect ultra-weak biological light, but can only detect biological macroscopic images, to go deep into the cell, molecular level, there must be a micro-photon counting imaging system. The difference between the two is that the photon-counting imaging system is a noise-limited system. The microscopic photon counting imaging system reported in this paper uses a 14 C isotope light source to monitor the state of the system and to ensure limit detection. The system can be used to study the distribution and function of trace biomolecules, to show the distribution of calcium ions in and out of cells, reactive oxygen species, monitoring of gene expression, and the like. From single photons to single molecules, the transformation from histological images to functional images will be an important development and provide a bright prospect for the application of biological medicine.