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在湖北省襄樊、恩施等主要烟区采集烟草黑胫病病株样本111个,分离纯化出30个烟草黑胫病菌株。采用游动孢子悬浮液注射接种鉴别寄主和TTZ(2,3,5-氯化三苯基四氮唑)颜色反应两种方法鉴定其生理小种类型。结果表明,供试的30个菌株在TTZ培养基中的反应皆为红色,有24个菌株在鉴别寄主反应中对L8和NC1071有较强致病力,而对N.nesophila无致病力,应为1号生理小种,占供试菌系的80%,为湖北省优势生理小种;其他6个菌株中有4个菌株对NC1071有较强致病力,但对L8致病力较弱,有1个菌株对L8和NC1071致病力较弱;1个菌株对NC1071致病力较弱,但对L8有较强致病力;6个菌株对N.nesophila无致病力,是否为2号生理小种还是其他致病型,尚待进一步研究确定。
A total of 111 tobacco black shank diseased samples were collected from major tobacco-growing areas in Xiangfan and Enshi in Hubei Province. Thirty tobacco black shank strains were isolated and purified. Two kinds of methods of zoospore suspension inoculation identification host and TTZ (2,3,5-triphenyltetrazolium) color reaction were used to identify their ecotypes. The results showed that the responses of the 30 tested strains in the TTZ medium were all red, 24 strains had stronger virulence to L8 and NC1071 in discriminating host reactions, but no virulence to N. neophila, Should be No. 1 race, accounting for 80% of the tested strains, for the dominant race in Hubei Province; other strains of four strains of NC1071 have a stronger virulence, but the virulence of L8 1 strain was weaker on L8 and NC1071; 1 strain had weaker virulence on NC1071 but stronger virulence on L8; 6 strains had no virulence to N. neophila, Physiological race 2 or other pathogenic type, yet to be further studied to determine.