论文部分内容阅读
用碱提酸沉法,以HPLC法测定穗花衫双黄酮的质量分数为指标,设计单因素和正交实验,确定了从卷柏中提取酸性成分的最佳工艺条件:ρ(NaOH)=2mg/mL水溶液为提取溶液、m(提取溶液):m(卷柏)=40:1、提取温度50℃、提取时间30min、沉降pH=3、提取1次,所得提取物中穗花衫双黄酮的质量分数稳定为10～12。该文首次报道了卷柏具有体内降血脂和体外抑制α-葡萄糖苷酶活性的功效。与模型组比较,酸性提取物能显著降低高脂血症小鼠的TC和LDL,极显著升高HDL;体外抑制α-葡萄糖苷酶活性(IC50=154.49μg/mL)高于对照阿卡波糖(IC50=1081.27μg/mL)。该文报告工作的新颖性已为河南大学图书馆2008年7月2日出具的第CX2008004号《科技查新报告》所证实。
Alkaline extraction and acid precipitation method was used to determine the mass fraction of spikelet and flavanone by HPLC method. Single factor and orthogonal experiments were designed to determine the optimum conditions for the extraction of acidic components from Selaginella: ρ(NaOH)=2mg /mL aqueous solution for the extraction solution, m (extraction solution): m (Selaginella) = 40:1, extraction temperature 50 °C, extraction time 30min, sedimentation pH = 3, extraction 1, the resulting extract of pansy flavonoids The quality is stable at 10-12. This article reports for the first time that selaginella has the effect of reducing blood fat in vivo and inhibiting α-glucosidase activity in vitro. Compared with the model group, acidic extracts can significantly reduce TC and LDL in hyperlipidemic mice, and significantly increase HDL; inhibition of α-glucosidase activity in vitro (IC50=154.49 μg/mL) is higher than that of control Akapo Sugar (IC50=1081.27 μg/mL). The novelty of the report has been confirmed by the “Science and Technology Innovation Report” No. CX2008004 issued by the Henan University Library on July 2, 2008.