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目的:探讨特异性核基质结合区结合蛋白1(SATB1)和微小RNA(microRNA，miR)-495-3P在甲状腺乳头状癌(PTC)侵袭和转移中的作用。方法:2019年9月至2020年4月，福建医科大学附属第二医院甲状腺乳腺外科采集肿瘤标本，将取得的40对PTC组织作为实验组，与之对应40对癌旁正常组织作为对照组。采用实时定量反转录聚合酶链反应(RT-qPCR)检测40对PTC组织及癌旁正常组织中SATB1和miR-495-3p的表达水平，用蛋白质印迹法(Western blot)检测16对PTC组织及癌旁正常组织中SATB1蛋白的表达水平。用si-SATB1转染人甲状腺乳头状癌细胞(TPC)-1后，用RT-qPCR、Western blot检测TPC-1中SATB1、miR-495-3p的表达水平，并用Pearson分析法进行相关性分析，转染si-NC的TPC-1为阴性对照组，转染si-SATB1的TPC-1为实验组。分别用细胞计数试剂盒(CCK-8)法、流式细胞计数、Trsnawell法检测敲低SATB1的表达对TPC-1增殖、凋亡、周期、侵袭、迁移能力的影响。两样本比较采用n t检验。n 结果:与癌旁正常组织比较，SATB1 mRNA和SATB1蛋白在PTC组织中呈高表达(1.27±0.14比0.86±0.23，n t＝8.484，n P<0.01；0.94±0.10比0.37±0.15，n t＝11.890，n P<0.01)，差异有统计学意义，miR-495-3p呈低表达(0.78±0.11比1.37±0.64，n t＝5.741，n P<0.01)，差异有统计学意义。SATB1和miR-495-3p的异常表达均与PTC淋巴结转移明显相关(1.34±0.14，n t＝2.576，n P<0.05；0.74±0.07，n t＝2.187，n P<0.05)。PTC中SATB1和miR-495-3p的表达水平呈负相关(n r＝-0.497，n P<0.01)。干扰TPC-1中SATB1的表达会导致miR-495-3p的表达水平高于阴性对照组(8.59±0.16比1.01±0.02，n t＝81.420，n P<0.01)，并且抑制细胞的增殖能力(0.39±0.01、0.52±0.01、0.58±0.03比0.43±0.01、0.60±0.01、0.72±0.01，n t＝4.899、9.798、7.668，n P<0.01)、促进细胞凋亡[(42.8±2.1)%比(7.6±0.7)%，n t＝27.540，n P<0.01]、减弱细胞侵袭(75.33±3.51比206.33±5.51，n t＝34.730，n P<0.01)、迁移(202.00±7.81比528.33±5.03，n t＝60.840，n P<0.01)能力并发生Gn 2/M周期阻滞[(36.7±1.2)%比(15.6±0.9)%，n t＝24.360，n P<0.01]，差异均有统计学意义。n 结论:miR-495-3p可能作为1种调节因子和SATB1共同参与了PTC侵袭、转移的过程。“,”Objective:To study the role of special adenine-thymine-rich-binding rich special AT-rich-binding protein 1 (SATB1) and microRNA (miRNA, miR)-495-3P in the invasion and metastasis of papillary thyroid carcinoma (PTC).Methods:All specimens were collected from department of Thyroid and Breast Surgery, Second Affiliated Hospital of Fujian Medical University from September 2019 to April 2020, including 40 cases of PTC and 40 cases of corresponding normal thyroid tissues. There were 10 males and 30 females, aged 18-68 years, with a median age of 43 years. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression levels of SATB1 and miR-495-3p in 40 pairs of PTC tissues and normal tissues adjacent to cancer. Western blotting was used to detect the expression levels of SATB1 protein in 16 pairs of PTC tissues and normal tissues adjacent to cancer. After transfecting Human thyroid papillary carcinoma cells (TPC-1) with si-SATB1, RT-qPCR and Western blotting were used to detect the expression levels of SATB1 and miR-495-3p in TPC-1, and Pearson analysis method was used for correlation analysis. Methyl thiazol tetrazolium(MTT) method, flow cytometry and Trsnawell method were used to detect the effect of knocking down SATB1 expression on TPC-1 proliferation, apoptosis, cycle, invasion and migration.Results:Compared with normal tissues adjacent to cancer, SATB1 mRNA and SATB1 proteins were highly expressed in PTC tissues (1.27±0.14 vs. 0.86±0.23, n t=8.484, n P<0.01 and 0.94±0.10 vs. 0.37±0.15,n t=11.890, n P<0.01), and miR-495-3p was down-regulated (0.78±0.11 vs. 1.37±0.64,n t=5.741, n P<0.01). The abnormal expression of SATB1 and miR-495-3p was significantly correlated to PTC lymph node metastasis (1.34±0.14,n t=2.576, n P<0.05 and 0.74±0.07,n t=2.187, n P<0.05). The expression levels of SATB1 and miR-495-3p in PTC were negatively correlated (n r=-0.497, n P<0.01). Interfering with the expression of SATB1 in TPC-1 could increase the expression level of miR-495-3p (8.59±0.16 vs. 1.01±0.02,n t=81.420, n P<0.01), and inhibit cell proliferation (0.39±0.01, 0.52±0.01, 0.58±0.03 vs. 0.43±0.01, 0.60±0.01, 0.72±0.01,n t=4.899, 9.798, 7.668, n P<0.01), promote cell apoptosis [(42.8±2.1)% vs. (7.6±0.7)%,n t=27.540, n P<0.01], weaken cell invasion (75.33±3.51 vs. 206.33±5.51,n t=34.730, n P<0.01), migration (202.00±7.81 vs. 528.33±5.03,n t=60.840, n P<0.01) and Gn 2/M cycle block occurred [(36.7±1.2)% vs. (15.6±0.9)%, n t=24.360, n P<0.01].n Conclusion:MiR-495-3p which may be used as a regulator factor and SATB1 jointly participate in the process of PTC invasion and metastasis.