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目的 对二氧化氯 (Cl O2 )破坏乙型肝炎表面抗原 (HBs Ag)抗原性的效果与戊二醛进行比较研究。方法 采用酶联免疫吸附实验 (EL ISA)进行检测。结果 Cl O2 消毒效果较戊二醛好 ,浓度 4 0 m g/L 时可将 HBs Ag抗原性破坏 ,戊二醛浓度则需为 2 0 0 mg/L ;p H值对 Cl O2 和戊二醛的消毒效果有影响 ,在酸性环境中 (p H值 6 .0时 ) ,Cl O2 的破坏效果最佳 ,碱性戊二醛则在碱性环境中 (p H值为 8.0时 )效果最强 ;2 0 %小牛血清即对 Cl O2 的消毒效果有明显影响 ,而 5 0 %以上的小牛血清才对戊二醛有影响。结论 Cl O2 是一种快速、高效的消毒剂
Objective To compare the effect of chlorine dioxide (Cl O2) on the antigenicity of hepatitis B surface antigen (HBs Ag) and glutaraldehyde. Methods The enzyme-linked immunosorbent assay (ELISA) was used to detect. Results The Cl O2 disinfection effect was better than that of glutaraldehyde. When the concentration was 40 mg / L, the antigenicity of HBsAg could be destroyed. The concentration of glutaraldehyde should be 200 mg / L. The value of p H for Cl O2 and glutaraldehyde , The effect of disinfection was the best in acid environment (p H = 6.0), and alkaline glutaraldehyde was the strongest in alkaline environment (p H = 8.0) ; 20% bovine serum that has a significant effect on the disinfection of Cl O2, and more than 50% of the bovine serum on glutaraldehyde only. Conclusion Cl O2 is a fast and highly effective disinfectant