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目的探索苯并(a)芘诱发小鼠实体瘤的情况及其对小鼠血清IL-2、IL-6的影响。方法 SPF级昆明种小鼠60只,雌雄各半,体重(20±2)g。随机分为三组:对照组、苯并(a)芘10 mg/kg组、苯并(a)芘20 mg/kg组(n=20),各组分别腹腔注射DMSO、10 mg/kg、20 mg/kg苯并(a)芘(注射体积0.1 ml/kg·bw),每日一次,连续10次。三个月后取血,ELISA测定血清IL-2及IL-6。取肝、肾、胃、肺,进行病理组织学检查。结果苯并(a)芘10 mg/kg组和20 mg/kg组,肝癌发生率为26.3%(5/19)和35.3%(6/17),胃癌发生率为0%(0/19)和41.2%(7/17);肾癌发生率为0%(0/19)和11.8%(2/17);癌前病变发生率,肝脏为73.7%(14/19)和64.7%(11/17),胃为68.4%(13/19)和29.4%(5/17),肾脏为47.4%(9/19)和58.8%(10/17)。实验各组癌前病变率均显著高于对照组(P<0.05);苯并(a)芘20 mg/kg组肝癌、胃癌、肾癌发生率均显著高于对照组(P<0.05);实验组肺脏未见明显损害。苯并(a)芘10 mg/kg组和20 mg/kg组血清IL-2、IL-6水平均显著低于对照组(P<0.01)。结论苯并(a)芘可诱发肝脏、胃、肾脏发生癌变或癌前病变,使小鼠血清IL-2、IL-6水平降低。
Objective To explore the situation of benzo (a) pyrene-induced solid tumor in mice and its effect on serum IL-2 and IL-6 in mice. Methods 60 SPF Kunming mice, male and female, weight (20 ± 2) g. The rats in each group were randomly divided into three groups: control group, benzo (a) pyrene 10 mg / kg group and benzo [a pyrene 20 mg / kg group 20 mg / kg benzo (a) pyrene (injection volume 0.1 ml / kg · bw) once daily for 10 consecutive times. Blood was taken three months later, and serum IL-2 and IL-6 were measured by ELISA. Take liver, kidney, stomach, lung, pathological examination. Results The incidence of hepatocellular carcinoma was 26.3% (5/19) and 35.3% (6/17) respectively, and the incidence of gastric cancer was 0% (0/19) in 10 mg / kg and 20 mg / And 41.2% (7/17) respectively. The incidence of renal cancer was 0% (0/19) and 11.8% (2/17) respectively. The incidence of precancerous lesions was 73.7% (14/19) and 64.7% / 17), the stomach was 68.4% (13/19) and 29.4% (5/17), the kidneys were 47.4% (9/19) and 58.8% (10/17). The incidence of precancerous lesions in each group was significantly higher than that in the control group (P <0.05). The incidence of liver cancer, gastric cancer and kidney cancer in the group of 20 mg / kg benzo (a) pyrene was significantly higher than that of the control group (P <0.05). No significant damage was found in the lungs of the experimental group. The serum levels of IL-2 and IL-6 in 10 mg / kg and 20 mg / kg benzo (a) Pyrene groups were significantly lower than those in the control group (P <0.01). Conclusions Benzo (a) pyrene can induce carcinogenesis or precancerous lesions in the liver, stomach and kidney, and decrease the serum levels of IL-2 and IL-6 in mice.