【摘 要】
：
SARS-CoV is a newly discovery pathogen causing severe acute respiratory problems.It has been established that the S protein in this pathogen plays an important
【机 构】
：
State Key Laboratory of Virology,College of Life Science,Wuhan University,Wuhan 430072,China
论文部分内容阅读
SARS-CoV is a newly discovery pathogen causing severe acute respiratory problems.It has been established that the S protein in this pathogen plays an important rule in the adsorption and penetration of SARS-CoV into the host cell by interaction with the ACE2 receptor.To determinant which functional motif of the S protein was involved in the interaction with ACE2,seven truncated S proteins deleted from the N or C terminal were obtained by an E.coli expression system and purified by column chromatography to homogeneity.Each truncated S protein was fixed on to the well of an ELISA plate and an interaction was initiated with the ACE2 protein.The adsorption were quantified by ELISA,and the results indicated that amino acids from 388 to 496 of the S protein was responsible for the interaction with the ACE2 receptor,and the interaction could be completely disrupted by an antibody specific to these amino acids.Deletions adjacent to this domain did not appear to have a significant impact on the interaction with ACE2,suggesting that the S protein of SARS-CoV could be developed as a vaccine to prevent the spread of SARS-CoV.
其他文献
目的:观察中药补益剂在介入疗法治疗中晚期大肠癌的临床疗效.方法:将接受治疗的55例中晚期大肠癌患者随机分为2组,对照组单纯接受介入治疗;治疗组在介入治疗的基础上辅以中药
目的:分析鼻内镜下手术致鼻中隔穿孔的原因,减少并发症的发生。方法:回顾鼻中隔患者456例,对于传统手术与内镜下手术及同期行鼻窦手术,发生鼻中隔穿孔的并发症进行比较、分析
采用计算机视觉识别技术对微小轴承端盖上的缺陷进行了自动识别。运用现代控制理论、图像采集与处理技术、软件编程技术,针对缺陷的位置、面积和深度所具有的随机性,设计了一套适合于检测微小轴承表面缺陷的系统。该系统可以采集到高质量的图像信息。利用SUSAN算子实现了对图像的分割和微小轴承表面缺陷的快速检测。实验结果表明,该方法的识别率可达到96%,并具有方法简单、定位准确、抗噪能力强、计算速度快、实时性好等
达涅利自动化部开发的最新的创新技术是通过轧机物流概念监视和调节机架间断面.该系统可沿着整个轧机对断面进行连续实时控制,以实现最佳张力控制.
利用ASI法和常规方法对宣城地区土壤速效P、K、Cu、Zn进行测定,结果表明2种方法呈极显著相关关系。
The soil available P, K, Cu and Zn in Xuancheng were determined by
讨论初始信任的涵义和分类,并利用海德平衡理论分析消费者对网上商店的初始信任。从事前因素、结构保证因素、初始信任和行为意图等方面构建消费者对网上商店的初始信任模型,
羟基磷灰石(HAP)是一种优质的医用生物材料,而纳米HAP更具有独特的性能。本文简要介绍了纳米HAP的独特结构和性能,并对近年来纳米HAP在肿瘤领域的研究进展进行综述。
Hydrox
外科治疗仍然是肝脏恶性肿瘤的首选治疗手段,不恰当的施行肝切除,有可能导致术后肝功能衰竭.超声、计算机X线断层扫描、磁共振及核医学等影像学检查已成为许多医学中心进行肝
人们对干扰素的研究是从发现病毒间的干扰现象开始的.对干扰素分类、受体、信号转导及抗病毒活性表达作用机理的了解,为临床应用干扰素抗病毒治疗开辟了广阔的前景.近年国内外有关人体基因多态性与药物疗效关系的研究成果不断涌现,现将其中有关干扰素诱导抗病毒蛋白的基因多态性与抗病毒疗效的关系做一综述。
目的 探讨三维调强适形放疗治疗食道癌的护理措施.方法 采用高能直线加速器6 mV-x线,实施全程三维调强适形放疗,2～3 Gy/次,5次/周,总剂量60 Gy.结果 42例患者除1例合并食道气管瘘停止放疗,1例并发肺炎中断治疗,其余均顺利完成治疗,不良反应小,总有效率92%.结论 护士在治疗过程中做好相应的护理,能提高患者对此新技术的认知程度。