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采用基因枪法把五个不同基因p35sGFP、pA ctG us、pU b iLuc、p35sPatP IV 4、pAH 2转移到玉米幼胚愈伤组织,以研究基因共转移的某些特性。试验结果表明,在处理B 1、B 2、B 3和B 4中,共获得转基因再生植株34株,分别涉及73、46、80和109个玉米幼胚愈伤组织,其基因转移频率分别是30.14%、17.39%、1.25%和2.75%。在基因转移中,一个幼胚愈伤组织一般可以获得一株转基因植株,同时也存在一个幼胚愈伤组织可以获得3株或以上的转基因植株的可能性。此外,有70%的机率能把3~4个不同基因进行至少一次克隆后共转移整合到玉米转基因再生植株上,而把五个不同基因共转移整合到玉米转基因再生植株上的机率则很低。不同基因的大小和质量会影响克隆的频率。已经整合到玉米转基因再生植株基因组上的转移基因的表达频率达到50%以上,不表达的转移基因则可能是基因沉默现象。
Five different genes, p35sGFP, pA ctG us, pU b iLuc, p35sPatP IV 4, and pAH2, were transferred to maize immature embryos using the gene gun method to study some of the characteristics of gene co-metastasis. The results showed that in the treatment of B 1, B 2, B 3 and B 4, a total of 34 transgenic plants were obtained, involving 73, 46, 80 and 109 maize immature embryos, the gene transfer frequency was 30.14%, 17.39%, 1.25% and 2.75%. In a gene transfer, an immature embryo callus can usually obtain one transgenic plant, and at the same time, there is a possibility that three or more transgenic plants can be obtained from immature embryo callus. In addition, there is a 70% chance of co-transfecting 3 to 4 different genes for at least one clonal transformation to maize GM plants, while the probability of co-transferring five different genes to maize GM plants is very low . The size and quality of different genes can affect the frequency of cloning. The frequency of transfer genes that have been integrated into the genome of transgenic corn plants has reached more than 50%, and the non-expressed genes may be gene silencing.