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香蕉花序在含BA0.5毫克/升+NAA0.5毫克/升+2,4-D2.0毫克/升的MS培养基上,雄花可以直接发生胚状体,诱导率为15％左右,花序轴和花苞片则先诱导出愈伤组织,然后分化胚状体。胚状体在含BA3.0毫克/升+NAA0.2毫克/升的MS培养基中同时处于黑暗培养,产生次级胚状体频率达90％以上,分化再生植株频率比光培养提高34.5％,降低培养基离子、激素、碳源浓度,可以加速再生植株成苗。移栽大田种植观察表明,不影响产量的小变异在2％以下。
Banana inflorescences were cultured on MS medium containing BA0.5 mg / L + NAA0.5 mg / L + 2,4-D2.0 mg / L and the male flowers could directly produce embryoid bodies with an induction rate of about 15% Axis and buds first induce callus and then differentiate into embryoid bodies. Embryoid bodies were cultured in dark medium in MS medium supplemented with BA3.0 mg / L and NAA0.2 mg / L at the same time. The frequency of secondary embryogenesis reached more than 90%, and the frequency of differentiated regenerated plants increased by 34.5% , Reduce medium ions, hormones, carbon source concentration, can accelerate the regeneration of plant seedlings. Transplanting field planting observation showed that small changes that do not affect the yield below 2%.