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为探索多成分、多途径干预急性脑缺血损伤的效果,用筛选出的中药复方-复方2为工具药(主要成分:人参皂苷,葛根黄酮,麦冬皂苷)进行研究。首先研究复方本身及其各组分对损伤的PC12细胞存活率和潜在机制,观察复方2对血栓形成和血小板聚集的影响,最后研究复方2对大鼠脑缺血再灌注损伤的影响。实验结果表明,复方2明显增强H2O2诱导的PC12细胞存活率,活性强于各组分;显著减少H2O2诱导的PC12细胞NO的产生,作用强于各组分;能增强SOD活性,其活性强于人参皂苷;降低MDA含量,其活性强于麦冬皂苷。体内实验表明,复方2能有效地抑制血小板聚集和静脉血栓形成。复方2皮下单剂量给药可明显减小脑缺血再灌注大鼠的脑梗死体积,并降低神经功能评分,表明复方2具有很强的抗脑缺血作用,这种作用可能是由多种成分通过多种途径同时干预而产生的。
In order to explore the effect of multi-component and multi-way intervention on acute cerebral ischemic injury, the selected Chinese traditional medicine compound-compound 2 was used as a tool (main components: ginsenoside, Pueraria flavonoids, Ophiopogon japonicus). First to study the compound itself and its components on the damage of PC12 cell survival rate and potential mechanism of compound 2 on the thrombosis and platelet aggregation observed the impact of compound 2 on cerebral ischemia-reperfusion injury in rats. The experimental results showed that compound 2 significantly enhanced the survival rate of PC12 cells induced by H2O2, and its activity was stronger than that of each component. The H2O2-induced NO production was significantly increased in PC12 cells, and its effect was stronger than that of each component. Ginsenosides; reduce MDA content, its activity is stronger than Ophiopogon japonicus. In vivo experiments show that compound 2 can effectively inhibit platelet aggregation and venous thrombosis. Compound 2 subcutaneous single-dose administration can significantly reduce the volume of cerebral infarction in cerebral ischemia-reperfusion rats, and lower neurological score, indicating that compound 2 has a strong anti-cerebral ischemia, this effect may be a variety of Components are produced by multiple interventions simultaneously.