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目的探讨血管内皮生长因子-C(VEGF-C)和血管内皮生长因子受体-3(VEGFR-3)在大肠癌中的表达,分析表达结果与肿瘤淋巴结转移及临床分期的关系。方法采用免疫组化SP法检测65例大肠癌及20例正常大肠黏膜中的VEGF-C和VEGFR-3的表达。结果65例大肠癌组织中VEGF-C和VEGFR-3阳性表达率分别为41.5%(27/65)和27.7%(18/65),正常黏膜表达率分别为15%(3/20)和0(0/20),肿瘤与正常黏膜比较,差异有统计学意义(P<0.05和P<0.01)。VEGF-C和VEGFR-3阳性表达率在有、无淋巴结转移组之间差异有统计学意义(P<0.01和P<0.05);两者的表达随临床分期升高而增多,其中VEGF-C的表达在不同临床分期组间差异有统计学意义(P<0.05);微淋巴管计数在VEGF-C表达阳性组和阴性组之间的差异有统计学意义(P<0.01)。结论VEGF-C和VEGFR-3在大肠癌中的表达与肿瘤淋巴结转移及临床分期的关系密切。
Objective To investigate the expression of vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) in colorectal cancer and to analyze the relationship between the expression and tumor lymph node metastasis and clinical stage. Methods Immunohistochemical SP method was used to detect the expression of VEGF-C and VEGFR-3 in 65 cases of colorectal carcinoma and 20 cases of normal colorectal mucosa. Results The positive rates of VEGF-C and VEGFR-3 in 65 cases of colorectal cancer were 41.5% (27/65) and 27.7% (18/65), respectively. The normal mucosa expression rates were 15% (3/20) and 0 (0/20). The difference between the tumor and the normal mucosa was statistically significant (P <0.05 and P <0.01). The positive rates of VEGF-C and VEGFR-3 were significantly different between those with and without lymph node metastasis (P <0.01 and P <0.05). The expression of VEGF-C and VEGFR-3 increased with the clinical stage, (P <0.05). The difference of lymphatic vessel count between VEGF-C positive group and negative group was statistically significant (P <0.01). Conclusion The expression of VEGF-C and VEGFR-3 in colorectal cancer is closely related to the metastasis of tumor and clinical stage.