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【目的】棉花叶片和非叶器官的结构和功能具有异质性,本文针对这一问题通过叶绿素荧光成像技术来研究叶片和非叶器官的光合异质性。【方法】本试验以陆地棉品种新陆早45号为材料,测定了叶绿素含量和叶绿素荧光参数等,以此探讨棉花叶片和非叶器官的光合特性和异质性。【结果】试验结果表明,棉花叶片及非叶器官均存在光合功能的异质性。通过比较不同器官的异质性表明,与叶片相比,非叶器官光合功能的变异程度较大;且不同参数的异质性随光照强度而变化。无论叶片还是非叶器官,与光化学过程相关的参数均随光照强度增加异质性变大,而与非光化学过程相关的参数均表现出随光照强度增加异质性变小的特点。【结论】在研究过程中,如使用普通光纤式叶绿素荧光仪来测定样品(叶片等)的光合性能时,需要对样品的不同部位进行多次测定,才能更好地反映所测定组织的光合性能。而如需要较精确的光化学猝灭或者非光化学猝灭相关的参数时,无论叶片还是非叶器官均需要加大测定的重复数来进行分析,以达到更为准确的目的。
【Objective】 The structure and function of cotton leaves and non-leaf organs are heterogeneous. In this paper, the photosynthetic heterogeneity of leaf and non-leaf organs was studied by chlorophyll fluorescence imaging technique. 【Method】 The chlorophyll content and chlorophyll fluorescence parameters of upland cotton variety “Xinluzao 45” were studied in this experiment to explore the photosynthetic characteristics and heterogeneity of cotton leaves and non-leaf organs. 【Result】 The results showed that there was heterogeneity of photosynthetic function in both cotton leaves and non-leaf organs. By comparing the heterogeneity of different organs, photosynthetic function of non-leaf organs was more variable than that of leaves. The heterogeneity of different parameters varied with light intensity. Regardless of leaf or non-leaf organs, the parameters associated with photochemical processes increased with increasing light intensity, whereas the parameters associated with non-photochemical processes showed a diminished heterogeneity with increasing light intensity. 【Conclusion】 In the process of research, such as the use of ordinary fiber chlorophyll fluorescence instrument to determine the photosynthetic properties of the samples (leaves, etc.), the need for multiple parts of the sample measured in order to better reflect the measured photosynthetic properties . For more accurate photochemical quenching or non-photochemical quenching related parameters, regardless of leaf or non-leaf organ need to increase the determination of the number of repetitions to be analyzed in order to achieve a more accurate purpose.