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目的 研究阳离子脂质体Ｌｉｐｏｆｅｃｔｉｎ（Ｌｉｐ）介导的ＤＮＡ 聚合酶α（ＤＮＡ－ ｐｏｌα） 反义寡核苷酸（ＡＳＯＤＮ）体外抑制人胃癌细胞的生长。方法 合成与人ｐｏｌ α催化亚基ｍＲＮＡ 翻译起始区１８ 个碱基互补的寡核苷酸（ＯＤＮ） ，与Ｌｉｐ 混合制备Ｌｉｐ＋ ＯＤＮ复合物，作用于ＭＧＣ－８０３、ＳＧＣ－７９０１ 人胃癌细胞２４ ｈ 后，细胞换液，继续培养２４ ｈ，用噻唑蓝（ＭＴＴ）比色法测细胞存活状态，流式细胞术（ＦＣＭ）分析癌细胞ＤＮＡ含量。结果 １０ μｇ／ｍｌｐｏｌα特异的ＡＳＯＤＮ在Ｌｉｐ 介导下可显著抑制人胃癌细胞的ＤＮＡ复制和细胞增殖，正义ＯＤＮ（ＳＯＤＮ） 无此作用。结论 ＤＮＡ－ ｐｏｌα特异ＡＳＯＤＮ可用于胃癌的基因治疗， 阳离子脂质体可促进ＡＳＯＤＮ大量进入癌细胞， 增强其生物效应。
Objective To study the inhibitory effect of cationic liposome Lipofectin (Lip) -mediated DNA polymerase α (ASODN) on the growth of human gastric cancer cells. Methods An oligonucleotide (ODN) complementary to 18 nucleotides of translation initiation region of human pol α catalytic subunit was synthesized and mixed with Lip to prepare Lip + ODN complex. The ODN complex was applied to MGC-803 and SGC-7901 human gastric cancer cells 24 h, the cells were replaced and continue to culture for 24 h. Cell viability was measured by MTT colorimetric assay. The DNA content of cancer cells was analyzed by flow cytometry (FCM). Results 10 μg / ml polα-specific ASODN could significantly inhibit the DNA replication and cell proliferation of human gastric cancer cells under the action of Lip, and no effect of ODN (SODN). Conclusion DNA-pol α-specific ASODN can be used in gene therapy of gastric cancer. Cationic liposomes can promote ASODN into large numbers in cancer cells and enhance its biological effects.