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近年来高效液相色谱法(HPLC)巳广泛应用于分离和测定各种金属离子与有机试剂的络合体系。本文以Tiron作为络合剂,用反相离子对色谱法,在紫外光谱区(254nm)同时测定钼和铼。结果表明,在进样量为20μl,钼的浓度范围在10.0—80.0μg/ml而铼在20.0—80.0μg/ml呈线性。变异系数(n=15)对于0.80μg的铼为1.42％,对于0.40μg的钼为1.86％。实验部分 1.仪器装置:WATERS,510型高压泵;730型数据处理机;441型UV/Vis检测器;
In recent years, high performance liquid chromatography (HPLC) has been widely used in the separation and determination of various metal ions and organic reagents complex system. In this paper, Tiron was used as a complexing agent to determine molybdenum and rhenium simultaneously in the UV spectral region (254nm) by reversed-phase ion-exchange chromatography. The results showed that the injection volume was 20 μl, the concentration of molybdenum was in the range of 10.0-80.0 μg / ml, and the rhenium was linear at 20.0-80.0 μg / ml. The coefficient of variation (n = 15) was 1.42% for 0.80 μg of rhenium and 1.86% for 0.40 μg of molybdenum. Experimental part 1. Instrument: WATERS, 510 high-pressure pump; Model 730 data processor; Type 441 UV / Vis detector;