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(Ⅰ)用于治疗胃-食管返流。本文报道一灵敏、可靠且专一的 HPLC 法测定生物体液中的(Ⅰ)和(Ⅱ),并应用此法研究其药动学。色谱条件 HPLC 色谱柱为 Nucleosil-C_(18)柱(颗粒直径3μm,10×4.6mm,id)。柱温50℃。荧光检测:激发波长270mm,发射波长370nm。流动相为含1ml 三乙胺的乙腈-1g/L 枸橼酸混合液(10∶90),使用前脱气处理,流速1.8 ml/min。0.1g/L的4-氨基-5-(甲氨磺酰基)-N-((卜烯丙基-2-吡咯烷基)-甲基)-2-甲氧苯甲酰胺(Ⅲ)的水溶液为内标。样品制备将1ml 血浆、全血或尿液置50ml 离心管中,加入内标(Ⅲ)50～100μl
(Ⅰ) for the treatment of gastric-esophageal reflux. This article reports a sensitive, reliable and specific HPLC method for the determination of (I) and (II) in biological fluids, and the application of this method to study its pharmacokinetics. Chromatographic conditions HPLC column Nucleosil-C_ (18) column (particle diameter 3μm, 10 × 4.6mm, id). Column temperature 50 ℃. Fluorescence detection: excitation wavelength 270mm, emission wavelength 370nm. The mobile phase consisted of acetonitrile-1g / L citric acid mixture (10:90) containing 1ml triethylamine and degassed before use with a flow rate of 1.8ml / min. An aqueous solution of 0.1 g / L of 4-amino-5- (methylsulfamoyl) -N - ((allyl-2-pyrrolidinyl) methyl) -2-methoxybenzamide (III) As an internal standard. Sample preparation Place 1 ml of plasma, whole blood or urine in a 50 ml centrifuge tube and add 50 ~ 100 μl of internal standard (Ⅲ)