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目的研究CCL2-2518位点基因多态性与儿童重症肠道病毒71型(EV71)感染的相关性。方法收集2014年5月至2015年9月在青岛大学附属医院儿科检测为EV71阳性的手足口病合并中枢神经系统感染患儿188例,根据病情轻重分为轻症组和重症组。同时选择正常体检儿童235例作为正常对照组。提取患儿外周血白细胞基因组DNA,用多重高温连接酶技术(i MLDR)检测EV71感染患儿及正常对照组CCL2-2518位点基因多态性,酶联免疫吸附法(ELISA)测定血清CCL2浓度。结果 EV71感染患儿与对照组相比,CCL2-2518位点各基因型分布及等位基因频率间差异无统计学意义(P>0.05)。重症组与轻症组相比,GG基因型和G等位基因频率更高(P<0.001)。EV71感染组血清CCL2浓度明显高于对照组(P<0.05),其中重症组CCL2浓度较轻症组及对照组均明显升高(P<0.05)。EV71感染组CCL2-2518位点各基因型间血清CCL2浓度比较:GG型高于AG型,AG型高于AA型,含G等位基因者(GG+AG)高于非G等位基因携带者(AA)(P<0.05)。结论 CCL2-2518位点G等位基因与EV71感染的严重程度相关,可能是EV71感染的易感因素。
Objective To investigate the association between CCL2-2518 locus polymorphism and childhood severe enterovirus 71 (EV71) infection. Methods From May 2014 to September 2015, 188 pediatric HFMD-infected children with central nervous system infection (EV71-positive) in pediatric department of Qingdao University Hospital were enrolled and divided into mild and severe group according to their severity. Meanwhile, 235 normal children were selected as the normal control group. Extracting peripheral blood leukocyte genomic DNA, ligase technology (i MLDR) with multiple high-temperature detection EV71 infection in children and normal control group CCL2-2518 gene polymorphism, enzyme-linked immunosorbent assay (ELISA) serum concentrations of CCL2 . Results There was no significant difference in genotype frequency and allele frequency between CCL2-2518 and EV71 children infected with EV71 (P> 0.05). The frequency of GG genotype and G allele was higher in severe group than in mild group (P <0.001). The serum CCL2 concentration in EV71 infection group was significantly higher than that in control group (P <0.05), and the CCL2 concentration in severe group was significantly higher than in mild group and control group (P <0.05). The levels of CCL2 in genotypes CCL2-2518 in EV71 infection group were higher than those in AG genotype, AG genotype was higher than AA genotype, G allele (GG + AG) was higher than non-G allele (AA) (P <0.05). Conclusion The G allele of CCL2-2518 locus correlates with the severity of EV71 infection and may be a predisposing factor for EV71 infection.