论文部分内容阅读
为探讨牛磺酸联合维生素E对小鼠酒精性肝损伤的保护效果,将10月龄清洁级雄性昆明小鼠60只按体重随机分为3组,分别为正常对照(生理盐水)组、酒精性肝损伤组、牛磺酸+维生素E干预组,每组20只。酒精性肝损伤组和牛磺酸+维生素E干预组每天7:00以50度白酒灌胃,牛磺酸+维生素E干预组于19:00以0.5mol/L的牛磺酸灌胃,灌胃量均为10ml/kg,每天1次;维生素E通过添加在普通饲料中进行补充(400mg/kg饲料);连续染毒2周。测定血中甘油三酯(TG)、丙二醛(MDA)、脂褐质(LF)的含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力,并对大鼠肝脏进行病理及免疫组化量化分析。结果显示,酒精性肝损伤小鼠血清TG、MDA和LF含量较高,SOD、GSH-Px活力均较低,差异有统计学意义(P<0.05)。而牛磺酸+维生素E能显著降低酒精性肝损伤小鼠血清TG、MDA和LF的含量,并增强血清中SOD以及GSH-Px的活力,差异有统计学意义(P<0.05)。病理学观察显示,牛磺酸+维生素E能干预可显著改善酒精对小鼠肝脏的损伤。免疫组化定量分析显示,酒精性肝损伤组面密度高于正常对照组和牛磺酸+维生素E干预组,差异均有统计学意义(P<0.05)。表明牛磺酸联合维生素E可能通过抗氧化的途径来减轻酒精对肝脏的损伤。
In order to investigate the protective effect of taurine combined with vitamin E on alcoholic liver injury in mice, 60 male Kunming mice of 10-month-old clean grade were randomly divided into three groups according to body weight: normal control (normal saline) group, alcohol Liver injury group, taurine + vitamin E intervention group, 20 rats in each group. Alcoholic liver injury group and taurine + vitamin E intervention group were orally administered with 50 degrees of white wine at 7:00 every day, taurine + vitamin E intervention group was orally administered with 0.5 mol / L taurine at 19:00, Amount of 10ml / kg, 1 times a day; vitamin E supplemented by adding ordinary feed (400mg / kg feed); continuous exposure for 2 weeks. The contents of triglyceride (TG), malondialdehyde (MDA), lipofuscin (LF) and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) , And pathological and immunohistochemical quantitative analysis of rat liver. The results showed that the contents of serum TG, MDA and LF were higher in alcoholic liver injury mice, and the activities of SOD and GSH-Px were lower in mice with alcoholic liver injury (P <0.05). However, taurine + vitamin E could significantly reduce the levels of serum TG, MDA and LF, and increase the activity of SOD and GSH-Px in alcoholic liver injury mice. The difference was statistically significant (P <0.05). Pathological observations showed that the taurine + vitamin E intervention can significantly improve alcohol damage in mice liver. Quantitative analysis by immunohistochemistry showed that the area density of alcoholic liver injury group was higher than that of normal control group and taurine + vitamin E intervention group, the differences were statistically significant (P <0.05). That taurine combined with vitamin E may be through the antioxidant pathway to reduce alcohol damage to the liver.