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目的研究氯化锰对大鼠生精细胞半胱天冬酶(Caspase-3)和抗霍奇金淋巴瘤细胞核抗体(Ki-67)表达的影响及意义。方法48只雄性SD大鼠随机分为6组:空白对照组,15和30mg/kgMnCl2组,锰与生理盐水均为腹腔注射,1次/d,5d/周,分别染锰4和6周。用免疫组化法检测睾丸生精细胞Caspase-3与Ki-67表达。结果与空白对照组比较,各染锰组Caspase-3阳性细胞率均显著升高(P<0.01);染锰剂量相同,染锰6周组与4周组比较,Caspase-3阳性细胞率均显著升高(P<0.01);染锰时间相同,30mg/kgMnCl2与15mg/kgMnCl2组比较,Caspase-3阳性细胞率均显著升高(P<0.01)。与空白对照组比较,染锰15mg/kgMnCl24周组Ki-67阳性细胞率降低(P<0.05);染锰30mg/kgMnCl24周组,15mg/kgMnCl26周组,30mg/kgMnCl26周组Ki-67阳性细胞率均显著降低(P<0.01);染锰时间相同,30mg/kgMnCl2组与15mg/kgMnCl2组比较,Ki-67阳性细胞率显著降低(P<0.01)。各组大鼠生精细胞Caspase-3阳性细胞率与Ki-67阳性细胞率呈明显负相关(r=-0.798,P<0.01)。结论染锰15mg/kg4周即可促进大鼠生精细胞Caspase-3表达和抑制大鼠生精细胞Ki-67表达,且存在一定的剂量-效应和时间-效应关系,锰的这种双重作用可能是导致大鼠生精障碍的主要机制。
Objective To study the effect of manganese chloride on the expression of spermatogenic cell caspase-3 and anti-Hodgkin’s lymphoma cell nuclear antigen (Ki-67) in rats. Methods 48 male Sprague-Dawley rats were randomly divided into 6 groups: blank control group, 15 mg / kg and 30 mg / kg MnCl2 group. Both manganese and normal saline were intraperitoneally injected once a day for 5 days and 4 weeks respectively. Immunohistochemistry was used to detect the expression of Caspase-3 and Ki-67 in spermatogenic cells of testis. Results Compared with the blank control group, the Caspase-3 positive rate in each manganese group increased significantly (P <0.01). Compared with the control group, the positive rate of Caspase-3 positive cells in the 6-day group (P <0.01). Compared with the same dose of manganese, the positive rate of Caspase-3 in 30mg / kg MnCl2 and 15mg / kg MnCl2 groups was significantly increased (P <0.01). Compared with the blank control group, the Ki-67 positive cells in the group of 24 mg MnCl2 treatment for 24 weeks (P <0.05), Ki-67 positive cells in the group of 30 mg / kg MnCl2 24 weeks, 15 mg / kg MnCl26 group and 30 mg / (P <0.01). The same time of manganese dying, the rate of Ki-67 positive cells decreased significantly in 30mg / kg MnCl2 group and 15mg / kg MnCl2 group (P <0.01). Caspase-3 positive rate of spermatogenic cells in each group was negatively correlated with Ki-67 positive rate (r = -0.798, P <0.01). Conclusions At the dose of 15 mg / kg manganese for 4 weeks, the expression of Caspase-3 in rat spermatogenic cells can be increased and the expression of Ki-67 in rat spermatogenic cells can be inhibited. There is a dose-effect and time- It may be the main mechanism of spermatogenesis in rats.