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1 材料 真武汤各组成药物购自济南建联药材店,经本院中药鉴定教研室鉴定,水煎醇提,浓缩成膏。鹌鹑由本院动物室提供。Beckman—700型全自动生化分析仪,美国贝克曼公司生产。2 方法 血清总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白——胆固醇(HDLC)含量用酶法测定。按公式计算动脉硬比指数(AsI)和抗动脉硬比比值(HDLC/TC)。动物处死,取主动脉,制切片,HE染色、光镜观察。3.结果 结果显示模型组鹌鹑的TC、TG水平及HDLC、AsI较正常组明显增高(TC:9.81±0.63/4.67±0.72;TG:3.52±0.81/1.56±0.63;LDLC:7.81±1.22
1 Materials Zhenwu Tang’s various components were purchased from Jinan Jianlian Herbal Medicine Store. They were identified by the Institute of Traditional Chinese Medicine Appraisal in this hospital, decocted with alcohol, and concentrated into a paste.鹌鹑 is provided by our animal room. Beckman-700 automatic biochemical analyzer, the United States Beckman production. 2 Methods Serum total cholesterol (TC), triglyceride (TG), and high-density lipoprotein-cholesterol (HDLC) levels were determined enzymatically. The arterial stiffness index (AsI) and anti arterial stiffness ratio (HDLC/TC) were calculated according to the formula. The animals were sacrificed and the aorta was removed and sectioned. HE staining and light microscopy were performed. 3. Results The results showed that the level of TC, TG and HDLC, AsI in the model group were significantly higher than those in the normal group (TC: 9.81±0.63/4.67±0.72; TG: 3.52±0.81/1.56±0.63; LDLC: 7.81±1.22;