论文部分内容阅读
观察硫酸锂对磷酸肌醇 3位羟基激酶LY2 940 0 2 (50 μmol· L-1)诱导体外培养的大鼠小脑颗粒神经元凋亡的拮抗作用 .结果显示 ,硫酸锂能明显阻断 LY 2 940 0 2诱导的神经元死亡 ,促进神经元存活 (MTT还原法 ) ,IC50 为 1 .2 5- 2 .5mmol· L-1.硫酸锂 (5.0 mmol· L-1)和 LY2 940 0 2同时处理神经元 ,LY2 940 0 2诱导的 DNA片段化(DNA琼脂糖凝胶电泳法 )和核固缩 (Hoechst332 58核染色法 )不再发生 .说明锂离子可对抗LY 2 940 0 2诱导的神经元凋亡 .采用间接荧光免疫法观察磷酸化 c- Jun蛋白免疫阳性神经元数目 ,结果显示 ,LY2 940 0 2组在 1 2 h可见大量磷酸化c- Jun免疫阳性神经元 ,2 4 h荧光更强 ;而硫酸锂(5.0 mmol·L-1)加 LY2 940 0 2组在各时间点未见磷酸化 c- Jun蛋白免疫阳性细胞 .提示锂离子抗LY2 940 0 2诱导的神经元凋亡的作用通过抑制c- Jun蛋白磷酸化过程起作用 ,其确切机理有待进一步探讨 .
To observe the antagonism of lithium sulfate on the apoptosis of cultured rat cerebellar granule neurons induced by phosphatidylinositol 3-hydroxylase LY2 940 0 2 (50 μmol·L-1) .The results showed that lithium sulfate could obviously block LY 2 940 0 2 induced neuronal death and promoted neuron survival (MTT reduction method) with IC50 of 12.5-2.5 mmol · L -1. Both lithium sulfate (5.0 mmol · L -1) and LY2 940 0 2 Treatment of neurons, LY2 940 0 2 induced DNA fragmentation (DNA agarose gel electrophoresis) and nuclear pyknosis (Hoechst332 58 nuclear staining) no longer occur.It shows that lithium ions can be against LY2 9402 induced neural The results showed that a large number of phosphorylated c-Jun immunoreactive neurons were found in LY2 940 0 2 group at 24 h, while the fluorescence at 24 h And no phosphorylation of c-Jun protein immunoreactive cells was observed at all time points in lithium sulphate 5.0 mmol·L-1 plus LY2 940 0 2 group, suggesting that lithium-induced apoptosis in neurons induced by LY2 940 0 2 The role of c-Jun by inhibiting the phosphorylation of protein plays a role in the exact mechanism to be further explored.