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本文使用特异的抗人T-和B-细胞血清间接免疫过氧化酶技术对31例患者乳腺癌组织中浸润的淋巴细胞亚群进行了鉴别。组织块约1×1×0.5cm,取自外科手术切除的乳腺癌标本的中央和边缘,在已烷中迅速冰冻(—70℃),用低温冰冻切片机切片(4μ),95%乙醇或丙酮固定3分钟,然后在室温中用兔抗人T-细胞血清或抗人B-细胞血清反应30分钟,冷的磷酸缓冲液漂洗后,与过氧化酶结合的羊抗免IgG 血清(1:20)反应30分钟,再用冷磷酸缓冲液漂洗三次共15分钟,在含3.3二氨基联苯胺的基质液中孵育3-6分钟。最
In this paper, we used the specific anti-human T- and B-cell serum indirect immunoperoxidase technology to identify infiltrating lymphocyte subsets in breast cancer tissue in 31 patients. The tissue blocks were approximately 1 x 1 x 0.5 cm, taken from the center and periphery of surgically resected breast cancer specimens, rapidly frozen in hexane (-70C), sectioned using a cryostat (4[mu]), 95% ethanol or Acetone was fixed for 3 minutes and then reacted with rabbit anti-human T-cell serum or anti-human B-cell serum for 30 minutes at room temperature. After rinsing with cold phosphate buffer, peroxidase-conjugated goat anti-IgG serum (1: 20) Reaction for 30 minutes, rinse three times with cold phosphate buffer for 15 minutes, and incubate for 3-6 minutes in a matrix solution containing 3.3 diaminobenzidine. most