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大豆胞囊线虫病3号生理小种(SCN3)是我国东北大豆产区的优势生理小种、危害较为严重,目前我国大豆生产上缺少抗病品种;然而常规育种方法培育抗性品种存在准确性差、遗传进程慢的缺点,因此分子辅助育种已成为培育抗性品种的有效途径之一。本研究根据前人的研究结果以抗大豆胞囊线虫病种质东农L-10为试验材料,克隆了抗病候选基因GmRSCN3-2的全长序列并构建了植物表达载体pCXSN-HA/GmRSCN3-2,进而利用发根农杆菌转化大豆材料来验证该候选基因的抗病性。结果表明:候选基因GmRSCN3-2在转基因植株阳性根内的平均雌虫数与空白对照植株根内的平均雌虫数存在显著差异,即GmRSCN3-2对大豆胞囊线虫病具有明显的抗性。
Soybean cyst nematode No. 3 physiological race (SCN3) is the dominant physiological races in northeastern soybean producing areas, the damage is more serious, the current lack of resistant varieties of soybean production in China; however, the conventional breeding methods to develop resistant varieties with poor accuracy , The slow genetic process, molecular-assisted breeding has become an effective way to cultivate resistant varieties. In this study, the full-length sequence of the resistance gene GmRSCN3-2 was cloned from the Dongnong L-10 germplasm resistant to soybean cyst nematode according to the results of previous studies and the plant expression vector pCXSN-HA / GmRSCN3 -2, and then Agrobacterium rhizogenes transformed soybean material to verify the candidate gene resistance. The results showed that there was a significant difference between the average number of female GmRSCN3-2 in the positive roots of transgenic plants and the average number of females in the control plants. That is, GmRSCN3-2 had obvious resistance to soybean cyst nematode.