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新生鼠发生缺氧缺血性脑损伤（HIBD） 后可出现神经细胞变性、坏死,还可诱发神经 细胞凋亡。采用原位末端标记技术（TUNEL 染色）可以检测发生凋亡的细胞核DNA片 段。已有资料报告在缺氧缺血性损伤后使用 硫酸镁可减轻大脑细胞损伤。本实验利用新 生鼠HIBD模型,观察硫酸镁预处理对新生 鼠神经细胞的保护作用。 材料和方法 实验对象为7日龄Wistar大鼠60只,体重12.5～17.0g,雌雄不限;新生鼠随机分为三组:正常对照组、缺氧缺血损伤组、硫酸镁
Neonatal rats hypoxic-ischemic brain damage (HIBD) can occur after degeneration and necrosis of nerve cells, but also induce neuronal apoptosis. Apoptotic nuclear DNA fragments can be detected by in situ terminal labeling (TUNEL staining). It has been reported that the use of magnesium sulfate after hypoxic-ischemic injury can reduce brain cell damage. In this study, neonatal rat HIBD model was used to observe the protective effect of magnesium sulfate preconditioning on the neurons of neonatal rats. MATERIALS AND METHODS: Sixty-seven-day-old Wistar rats weighing 12.5-17.0 g were randomly divided into three groups: normal control group, hypoxic ischemic injury group, magnesium sulfate