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与其他亚细胞结构相比,中心体由于其分离和纯化方面的困难使其蛋白质组学研究一直处于滞后状态.通过免疫荧光证明特异性识别中心体的6名自身免疫病人的自发抗血清被用来鉴定它们相应的抗原即中心体蛋白.利用细胞全蛋白的Western blot检测血清后,从Western blot膜上每条单独条带上洗脱结合的抗体,并确定产生中心体特异性荧光染色的抗体洗脱来源.通过免疫沉淀获得并通过质谱鉴定该抗体相应的抗原蛋白,共鉴定出6种中心体蛋白,包括2种已知的中心体蛋白和4种未知定位或未见中心体定位报道的蛋白.这些蛋白涉及细胞周期调控、信号转导通路、分子伴侣和代谢酶类,反映了中心体功能的多样性.
Compared with other subcellular structures, centrosomes have been delayed in their proteomics research due to their difficulties in isolation and purification, and the spontaneous antisera of six autoimmune patients that specifically recognize centrosomes were shown to be used by immunofluorescence To identify their corresponding antigen, centrobodies. After serum was detected by western blotting of whole cell proteins, the bound antibodies were eluted from each individual band on Western blot membranes and antibodies producing centrosome-specific fluorescence were identified Elution source.A total of 6 kinds of centromere proteins were identified and identified by mass spectrometry, including 2 kinds of known centrobodies and 4 kinds of unknown centromere or reported centrosomes These proteins are involved in cell cycle regulation, signal transduction pathways, chaperones and metabolic enzymes, reflecting the diversity of centrosome functions.