利用CCNU在过量酸中的脱亚硝基反应,建立以分光光度法测定体内微量CCNU含量的方法,并对其测试敏感性、可靠性进行分析讨论和评价。结果显示:在血液及脑组织中,CCNU浓度与吸收度间线性相关良好,可测浓度范围分别为0.2～40μg/ml和40～80μg/g。与既往方法相比,本法不失为一种快捷、可靠且敏感的测试方法。 Using CCNU in nitrosyl reaction in excess acid, a method of spectrophotometric determination of trace CCNU in vivo was established, and its sensitivity and sensitivity were analyzed and discussed. The results showed that there was a good linear correlation between CCNU concentration and absorption in the blood and brain tissue. The measurable concentrations ranged from 0.2 to 40 μg / ml and from 40 to 80 μg / g, respectively. Compared with the previous method, this method can be regarded as a quick, reliable and sensitive test method.