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目的:建立测定双黄连制剂中连翘苷含量的方法。方法:采用高效液相色谱法。直接稀释法制备供试品溶液,色谱柱为AgilentZorbaxSB-C18柱,柱温为35℃,流动相为乙腈-0.012mol.L-1醋酸钠溶液(v/v=23:77),流速为1mL.min-1,检测波长为277nm,进样量为10μL。结果:连翘苷进样量在0.11～5.5μg范围内与峰面积积分值呈良好的线性关系(r=0.9999),连翘苷平均回收率为98.8%,RSD=0.3%(n=6)。结论:改进后的方法简便、高效、准确、重复性好,可用于双黄连制剂的质量控制。
Objective: To establish a method for the determination of forsythin in Shuanghuanglian preparations. Methods: Using high performance liquid chromatography. The direct dilution method was used to prepare the test solution. The column was an Agilent Zorbax SB-C18 column with a column temperature of 35 ° C. and a mobile phase of acetonitrile-0.012 mol·L-1 sodium acetate solution (v / v = 23:77) . min-1, detection wavelength of 277nm, injection volume of 10μL. Results: The calibration curve of forsythin in the range of 0.11-5.5 μg showed a good linear relationship with the peak area (r = 0.9999), the average recovery of forsythin was 98.8% and RSD was 0.3% (n = 6) . Conclusion: The improved method is simple, efficient, accurate, reproducible and can be used for the quality control of Shuanghuanglian preparations.