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目的研究子宫颈炎组织和子宫颈鳞癌组织中MHC-Ⅰ类抗原呈递相关蛋白(TAP1、TAP2、β2-MG、HSP70)在维吾尔族和汉族妇女中的表达差异。方法应用免疫组化SP法和聚合酶链反应技术检测子宫颈炎组织以及子宫颈鳞癌组织中HPV抗原呈递相关蛋白TAP1、TAP2、β2-MG和HSP70的表达和HPV16的感染情况。结果TAP1、TAP2和β2-MG在子宫颈鳞癌组织中阳性表达率(51%、60%和61%),均低于子宫颈炎组织(77%、95%和96%,P均<0.05)。在子宫颈鳞癌组织中,维、汉族间TAP1、TAP2和β2-MG阳性表达有差异(P<0.05)。在子宫颈炎组织中,维、汉族间TAP1、β2-MG阳性表达有差异(P<0.05)。维吾尔族子宫颈鳞癌中β2-MG表达与HPV16感染关系为正相关(r=0.216,P<0.05)。汉族子宫颈鳞癌中,TAP2与β2-MG间存在正相关(P<0.05)。维吾尔族子宫颈鳞癌中,TAP1与β2-MG、TAP1与HSP70间存在正相关(P<0.05)。结论子宫颈鳞癌中TAP1、TAP2和β2-MG的表达量下降,导致细胞表面MHC-Ⅰ类分子表达降低,可能是肿瘤细胞逃逸免疫监视的一种机制,且在维吾尔族和汉族间相关性呈现差异,提示不同蛋白在不同民族间子宫颈鳞癌的发病机制方面可能存在不一致。
Objective To study the expression difference of MHC class Ⅰ antigen presenting protein (TAP1, TAP2, β2-MG, HSP70) in Uterine cervix and cervical squamous cell carcinoma in Uigur and Han women. Methods The expression of HPV antigen presenting protein TAP1, TAP2, β2-MG and HSP70 and HPV16 infection in cervicitis and cervical squamous cell carcinoma were detected by immunohistochemical SP method and polymerase chain reaction (PCR). Results The positive expression rates of TAP1, TAP2 and β2-MG in cervical squamous cell carcinoma (51%, 60% and 61%) were lower than those in cervicitis (77%, 95% and 96%, P <0.05 ). In cervical squamous cell carcinoma, the positive expression of TAP1, TAP2 and β2-MG were different between Uygur and Han nationality (P <0.05). In cervicitis tissues, the positive expression of TAP1 and β2-MG in Victoria and Han nationalities were different (P <0.05). Uygur cervical squamous cell carcinoma of the β2-MG expression and HPV16 infection was positively correlated (r = 0.216, P <0.05). Han cervical squamous cell carcinoma, there is a positive correlation between TAP2 and β2-MG (P <0.05). Uygur cervical squamous cell carcinoma, TAP1 and β2-MG, TAP1 and HSP70 there is a positive correlation (P <0.05). Conclusions The decreased expression of TAP1, TAP2 and β2-MG in squamous cell carcinoma of the cervix results in the decrease of MHC-Ⅰ expression on the cell surface, which may be a mechanism of tumor cell escape immune surveillance. The correlation between Uighur and Han populations Showing differences, suggesting that different proteins in different ethnic groups may be inconsistent with the pathogenesis of cervical squamous cell carcinoma.