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目的:制备高纯度的胡芦巴总皂苷。方法:采用乙醇回流法提取胡芦巴总皂苷,依次加正己烷、三氯甲烷、乙酸乙酯、正丁醇进行系统溶剂萃取,三氯甲烷有效部位经HPD-400型大孔吸附树脂富集纯化。采用显色反应和TLC鉴别总皂苷,运用UV测定胡芦巴总皂苷含量。结果:三氯甲烷萃取部位中总皂苷纯度40%,得率6.28%;水洗脱液中胡芦巴总皂苷纯度80.5%,得率0.32%。结论:优选的提取纯化工艺快速简便,为胡芦巴总皂苷的药效学评价奠定基础。
Objective: To prepare high purity fenugreek total saponin. Methods: The total saponin of fenugreek was extracted by ethanol refluxing, followed by systematic solvent extraction with n-hexane, chloroform, ethyl acetate and n-butanol. The effective fractions of trichloromethane were enriched by HPD-400 macroporous adsorption resin purification. The total saponin was identified by color reaction and TLC. The content of fenugreek saponin was determined by UV. Results: The purity of total saponin in chloroform extraction site was 40% with a yield of 6.28%. The purity of fenugreek saponin in water elution was 80.5% with a yield of 0.32%. Conclusion: The optimized extraction and purification process is rapid and simple, which lays the foundation for the pharmacodynamic evaluation of fenugreek total saponins.