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目的探讨N-乙酰半胱氨酸(NAC)在氢醌(HQ)抑制HL-60细胞向单核系分化过程中所起的作用。方法将NAC 1、2.5、5和10 mmol/分别与HQ 50μmol/L和豆蔻酰佛波乙酯(TPA)20 nmol/L共同处理细胞,处理48 h后收集细胞。通过硝基四氮唑蓝还原反应鉴定细胞分化;CCK-8检测细胞增殖;活性氧检测试剂盒鉴定活性氧的变化。结果与HQ和TPA+HQ组比较,随NAC剂量的增加可以引起细胞增殖的增加,差异具有统计学意义(P<0.01)。在HL-60细胞向单核系分化的过程中,与TPA+HQ组比较,细胞的分化能力随着NAC剂量的增加而增强,差异具有统计学意义(P<0.01),其中NAC 5和10 mmol/L作用最为显著。细胞内活性氧(ROS)检测显示,与空白对照组比较,HQ组和TPA+HQ组含量明显增强,差异有统计学意义(P<0.01);与TPA+HQ组比较,NAC各个剂量组ROS含量明显降低(P<0.01)。5个时间点测量细胞内活性氧含量差异有统计学意义(F=564.3,P<0.01)。结论 HQ能够抑制HL-60向单核系分化,这种抑制作用会随着NAC剂量的增加而降低,这种影响是通过清除细胞内活性氧来实现的。
Objective To investigate the role of N-acetylcysteine (NAC) in inhibiting the differentiation of HL-60 cells into mononuclear cells by hydroquinone (HQ). Methods The cells were co-treated with NAC at 1, 2.5, 5 and 10 mmol / L and 50 nmol / L HQ and 20 nmol / L TPA for 48 h. Cell differentiation was identified by reduction of nitrotetrazolium blue; cell proliferation was assayed by CCK-8; and change of reactive oxygen species by reactive oxygen detection kit. Results Compared with HQ and TPA + HQ groups, the increase of NAC dose could increase the cell proliferation, the difference was statistically significant (P <0.01). Differentiation of HL-60 cells into mononuclear cells showed that compared with TPA + HQ group, the differentiation ability of cells increased with the increase of NAC dosage (P <0.01), of which NAC 5 and 10 The effect of mmol / L is the most significant. Compared with the control group, the content of ROS in the HQ group and the TPA + HQ group was significantly increased (P <0.01). Compared with the TPA + HQ group, the levels of ROS Content was significantly lower (P <0.01). There were significant differences in reactive oxygen species (F = 564.3, P <0.01) at 5 time points. Conclusions HQ can inhibit the differentiation of HL-60 to monocytogenes. This inhibition may be reduced with the increase of NAC dosage, which is achieved through the removal of intracellular reactive oxygen species.