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目的:建立治带片的质量控制方法。方法:采用薄层色谱法对治带片中的苍术、金樱子进行薄层鉴别,采用高效液相色谱法对治带片中苦参碱和氧化苦参碱进行含量测定。结果:薄层色谱中,色谱斑点清晰,阴性无干扰;测得的苦参碱和氧化苦参碱质量分别在0.33~3.3μg(r=0.999 8)和0.038~0.38μg(r=0.999 9)时与色谱峰面积之间线性关系良好,二者平均回收率分别为100.4%、101.5%,RSD分别为1.4%、1.5%。结论:该方法简便、快速、准确,可作为治带片质量控制的一个有效方法。
Objective: To establish a method of quality control of gingiva tablets. Methods: Thin layer chromatography was used to identify Atractylodes lanceolata and Rosa laevigata in the strip. The contents of matrine and oxymatrine in the strip were determined by high performance liquid chromatography. Results: The chromatographic spots were clear and negative in the TLC. The measured concentrations of matrine and oxymatrine ranged from 0.33 to 3.3 μg (r = 0.999 8) and from 0.038 to 0.38 μg (r = 0.999 9) The linear relationship between peak area and chromatographic peak area was good. The average recoveries were 100.4% and 101.5%, respectively, with RSDs of 1.4% and 1.5%, respectively. Conclusion: The method is simple, rapid and accurate and can be used as an effective method for the quality control of banding tape.