论文部分内容阅读
目的使用增殖细胞核抗原反义寡脱氧核苷酸（ＰＣＮＡ ＡＳＯＤＮ）作用于牛晶状体上皮细胞，观察其对细胞增殖活性的影响，以探讨ＰＣＮＡ ＡＳＯＤＮ技术防治后发性白内障形成的新途径。方法应用３Ｈ－ＴｄＲ掺入法检测细胞增殖活性，用流式细胞仪检测晶状体上皮细胞 ＰＣＮＡ蛋白的表达。结果 ＰＣＮＡ ＡＳＯＤＮ ３０～５０ μｍｏｌ／Ｌ可使细胞增殖显著受抑制，其 ＣＰＭ值分别从对照组的 ６ ６９９． ６７下降为 ５ ９８６． ３３，５ ５２５． ３３和 ５ １５４． ６７，ＰＣＮＡ蛋白在晶状体上皮细胞的表达阳性率下调至１２．３２％，与对照组比较有显著性差异（Ｐ＜０．０１）。ＰＣＮＡ ＳＯＤＮ组则作用不明显，与对照组比较无显著性差异（Ｐ＞０．０５），而ＰＣＮＡ ＡＳＯＤＮ组与ＰＣＮＡ ＳＯＤＮ组比较差异显著（Ｐ＜０．０１）。结论ＰＣＮＡ ＡＳＯＤＮ可以显著抑制牛晶状体上皮细胞体外增殖活性，其抑制作用可能通过阻断ＰＣＮＡ ｍＲＮＡ的翻译过程而影响 ＰＣＮＡ蛋白表达。
Objective To investigate the effect of proliferating cell nuclear antigen antisense oligodeoxynucleotide (PCNA ASODN) on bovine lens epithelial cells (PCNA) to observe the effect of PCNA ASODN on cell proliferative activity. Methods 3H-TdR incorporation assay was used to detect cell proliferation. The expression of PCNA protein in lens epithelial cells was detected by flow cytometry. Results PCNA ASODN 30 ~ 50 μmol / L significantly inhibited cell proliferation, the CPM values were 6 699 in the control group. 67 dropped to 5 986. 33,5 525. 33 and 5 154. 67. The positive expression rate of PCNA protein in lens epithelial cells was reduced to 12.32%, which was significantly different from that in control group (P <0.01). There was no significant difference between the PCNA SODN group and the control group (P> 0.05), but there was significant difference between the PCNA ASODN group and the PCNA SODN group (P <0.01). Conclusion PCNA ASODN can significantly inhibit the proliferation of bovine lens epithelial cells in vitro, and its inhibitory effect may affect the expression of PCNA by blocking the translation of PCNA mRNA.